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Sarcoramphus papa

ABSTRACT: Sarcoramphus papa overview

PROVIDER: PRJNA846465 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR19167646_1.fastq.gz	Fastqsanger.gz
	SRR19167646_2.fastq.gz	Fastqsanger.gz

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Sarcoramphus papa

Project description:Sarcoramphus papa

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Project description:Sarcoramphus papa (king vulture) genome, bSarPap1, haplotype 1

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Sarcoramphus papa isolate:bSarPap1

Project description:Sarcoramphus papa (king vulture) genome, bSarPap1, haplotype 2

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Dysregulated neutrophil responses and neutrophil extracellular trap formation and degradation in PAPA syndrome

Project description:Pyogenic arthritis, pyoderma gangrenosum and acne (PAPA) syndrome is characterized by flares of sterile arthritis with neutrophil infiltrate and the overproduction of Interleukin (IL)-1β. The purpose of this study was to elucidate the potential role of neutrophil subsets and neutrophil extracellular traps (NETs) in the pathogenesis of PAPA. The transcriptome of PAPA normal-dense neutrophils, autologous LDGs, and healthy control normal-dense neutrophils was characterized using RNASeq. The transcriptome of a PAPA skin biopsy and a healthy control skin sample was elucidated using RNASeq.

2018-10-23 | GSE117168 | GEO

Project description:Sarcoramphus papa (king vulture) genome, bSarPap1

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Project description:Sarcoramphus papa (king vulture) genome, bSarPap1, sequence data

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Project description:Dysregulated neutrophil responses and neutrophil extracellular trap formation and degradation in PAPA syndrome

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The Effects of Nitric Oxide on Choroidal Gene Expression

Project description:We determined the effect of nitric oxide on chick choroids using the NO donor PAPA-NONOate

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Heterologous expression of pediocin/papA in Bacillus subtilis.

Project description:Listeria monocytogenes is a food-borne pathogen. Pediocin is a group IIα bacteriocin with anti-listeria activity that is naturally produced by Pediococcus acidilactic and Lactobacillus plantarum. The pedA/papA gene encodes pediocin/plantaricin. In native hosts, the expression and secretion of active PedA/PapA protein rely on the accessory protein PedC/PapC and ABC transporter PedD/PapD on the same operon. The excretion machines were also necessary for pediocin protein expression in heterologous hosts of E. coli, Lactobacillus lactis, and Corynebacterium glutamicum. In this study, two vectors carrying the codon sequence of the mature PapA peptide were constructed, one with and one without a His tag. Both fragments were inserted into the plasmid pHT43 and transformed into Bacillus subtilis WB800N. The strains were induced with IPTG to secrete the fused proteins PA1 and PA2. Supernatants from both recombinant strains can inhibit Listeria monocytogenes ATCC54003 directly. The fused protein possesses inhibition activity as a whole dispense with removal of the leading peptide. This is the first report of active pediocin/PapA expression without the assistance of PedCD/PapCD in heterogeneous hosts. In addition, the PA1 protein can be purified by nickel-nitrilotriacetic acid (Ni-NTA) metal affinity chromatography.

| S-EPMC9148482 | biostudies-literature

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