Project description:Levilactobacillus brevis strains isolated from various dairy products (artisanal Greek Feta and Kefalograviera cheeses, and raw sheep milk)
| PRJNA847016 | ENA
Project description:Lactiplantibacillus plantarum strains isolated from various dairy products (artisanal Greek Feta and Kefalograviera cheeses, and raw sheep milk)
| PRJNA846557 | ENA
Project description:Weissella paramesenteroides strains isolated from various dairy products (artisanal Greek Feta and Kefalograviera cheeses, and raw sheep milk)
| PRJNA847013 | ENA
Project description:Leuconostoc mesenteroides strains isolated from various dairy products (artisanal Greek Feta and Kefalograviera cheeses, and raw sheep milk)
| PRJNA847019 | ENA
Project description:Pediococcus pentosaceus strains isolated from various dairy products (artisanal Greek Feta and Kefalograviera cheeses)
Project description:Milk and dairy products are an essential food and an economic resource in many countries. Milk component synthesis and secretion by the mammary gland involve expression of a large number of genes whose nutritional regulation remains poorly defined. We aim at understanding the genomic influence on milk quality and synthesis by comparing two sheep breeds, with different milking attitude, Sarda and Gentile di Puglia, using sheep-specific microarray technology. From sheep ESTs deposited at NCBI, we generated the first annotated microarray developed for sheep with a covering of most of the genome.
Project description:Milk and dairy products are an essential food and an economic resource in many countries. Milk component synthesis and secretion by the mammary gland involve expression of a large number of genes whose nutritional regulation remains poorly defined. We aim at understanding the genomic influence on milk quality and synthesis by comparing two sheep breeds, with different milking attitude, Sarda and Gentile di Puglia, using sheep-specific microarray technology. From sheep ESTs deposited at NCBI, we generated the first annotated microarray developed for sheep with a covering of most of the genome. Whole tissue samples of mammary gland were collected from 4 lactating individuals of two sheep (Ovis aries) breeds, Gentile di Puglia and Sarda. Biopsies of lactating mammary tissue were taken at two lactation stages (first record, stage 01: 6 days after lambing; second record, stage 02: 44 days after lambing) in both breeds. Tissues from mammary gland were immersed in RNAlater (Sigma) immediately after biopsy and stored at -20°C.
Project description:Previous work has demonstrated that elevated maternal lipid intake (particularly from dairy products) is associated with increased lipids and altered fatty acid profile in milk produced by healthy lactating women. We investigate our primary hypothesis that a maternal diet rich in full-fat dairy products would simultaneously increase milk lipid percent and expression of genes related to the uptake and/or de novo biosynthesis of milk lipids.
Project description:To investigate the molecular bases of diet induced differences in milk composition, we collected milk from mid lactation dairy ewes and after 3 weeks of diet supplementation with extruded linseed. RNAs were isolated from milk somatic cells isolated from milk of 3 sheep and Illumina RNA sequencing was performed to analyze RNA synthesis in these cells.
