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Identification of differentially expressed microRNAs as potential biomarkers for carcinoma ex pleomorphic adenoma

ABSTRACT: Identification of differentially expressed microRNAs as potential biomarkers for carcinoma ex pleomorphic adenoma

PROVIDER: PRJNA847079 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19578910.fastq.gz	Fastqsanger.gz
	SRR19578911.fastq.gz	Fastqsanger.gz
	SRR19578912.fastq.gz	Fastqsanger.gz
	SRR19578913.fastq.gz	Fastqsanger.gz
	SRR19578914.fastq.gz	Fastqsanger.gz

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Identification of differentially expressed microRNAs as potential biomarkers for carcinoma ex pleomorphic adenoma.

Project description:Carcinoma ex pleomorphic adenoma (CXPA) is a rare malignancy that transforms from PA. Early detection of the carcinoma by biopsy is difficult due to similar histopathology of the malignant and benign components. To address this, we investigated and compared the characteristic miRNA expression patterns across samples of the PA, carcinomatous portions (CA) of CXPA, as well as conventional PA. We selected 13 CXPA and 16 conventional PA FFPE samples, separated the PA and CA portions of CXPA samples and conducted miRNA profiling for each group. Among 13 transcripts that were differentially expressed between PA and CA of CXPA, eight miRNAs were up-regulated and five down-regulated in CA. Bioinformatic analysis revealed that the up-regulated miRNAs were related to cancer progression and down-regulated ones to tumor suppression. Additionally, seven miRNAs were significantly up-regulated in PA of CXPA compared to conventional PA, although they are histopathologically similar. Almost all of these transcripts interacted with TP53, a well-known tumor suppressor. In conclusion, we identified differentially expressed miRNAs in PA and CA of CXPA, which were closely associated with TP53 and various cancer-related pathways. We also identified differentially expressed miRNAs in the PA of CXPA and conventional PA which may serve as potential biomarkers.

| S-EPMC9352753 | biostudies-literature

Analysis of mRNA expression in pleomorphic adenoma

Project description:We identified transcriptional landscape in pleomorphic adenoma (PA).

2024-07-14 | GSE180105 | GEO

Gene fusion detection of salivary gland pleomorphic adenoma

Project description:Gene fusion detection of salivary gland pleomorphic adenoma

2021-05-31 | E-MTAB-9818 | biostudies-arrayexpress

Landscape of N6-methyladenosine modification patterns in invasive malignant pleomorphic adenoma

Project description:N6-methyladenosine (m6A) is one of the most popular RNA modifications, which is widely found in messenger RNAs (mRNAs) .In our study,we provide m6A profiles of human invasive malignant pleomorphic adenoma, which open an avenue for in-depth knowledge and understanding of m6A topology in invasive malignant pleomorphic adenoma.

2021-03-10 | GSE161879 | GEO

Analysis of mRNA expression in invasive malignant pleomorphic adenoma

Project description:Invasive malignant pleomorphic adenoma (IMPA) results from the malignant transformation of pleomorphic adenoma (PA). The former is a high-grade malignant tumor, whereas the latter is a benign opposite. Study on the molecular mechanism in the progression of PA to IMPA will be of benefit to elucidate the reasons for different biological behaviors among these salivary gland tumors with the same origin. But there is no valuable and non-invasive biomarker to screen IMPA currently. Studies showed many salivary molecules can detect several systemic diseases. We aimed to investigate whether salivary mRNAs (mRNA) can act as a biomarker to detect IMPA.

2022-01-12 | GSE179895 | GEO

Identification of differentially expressed microRNAs as potential biomarkers for carcinoma ex pleomorphic adenoma

Project description:We investigated and compared the characteristic miRNA expression patterns across samples of the PA, carcinomatous portions (CA) of CXPA, as well as conventional PA.

2022-06-13 | GSE205670 | GEO

Analysis of mRNA expression in pleomorphic adenoma

Project description:Analysis of mRNA expression in pleomorphic adenoma

| PRJNA746564 | ENA

Gene fusion detection of salivary gland pleomorphic adenoma

Project description:Gene fusion detection of salivary gland pleomorphic adenoma

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Analysis of mRNA expression in invasive malignant pleomorphic adenoma

Project description:Analysis of mRNA expression in invasive malignant pleomorphic adenoma

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Quantitative promoter methylation differentiates carcinoma ex pleomorphic adenoma from pleomorphic salivary adenoma.

Project description:BACKGROUND: potential epigenetic biomarkers for malignant transformation to carcinoma ex pleomorphic adenoma (Ca ex PSA) have been sought previously with and without specific comparison with the benign variant, pleomorphic salivary adenoma (PSA). Previous analysis has been limited by a non-quantitative approach. We sought to demonstrate quantitative promoter methylation across a panel of tumour suppressor genes (TSGs) in both Ca ex PSA and PSA. METHODS: quantitative methylation-specific real-time polymerase chain reaction (qMSP) analysis of p16(INK4A), CYGB, RASSF1, RAR?, human telomerase reverse transcriptase (hTERT), Wilms' tumour 1 (WT1) and TMEFF2 gene promoters was undertaken on bisulphite-converted DNA, previously extracted from archival fixed tissue specimens of 31 Ca ex PSA and an unrelated cohort of 28 PSA. All target regions examined had formerly been shown to be hypermethylated in salivary and/or mucosal head and neck malignancies. RESULTS: the qMSP demonstrated abnormal methylation of at least one target in 20 out of 31 (64.5%) Ca ex PSA and 2 out of 28 (7.1%) PSA samples (P<0.001). RASSF1 was the single gene promoter for which methylation is shown to be a statistically significant predictor of malignant disease (P<0.001) with a sensitivity of 51.6% and a specificity of 92.9%. RAR?, TMEFF2 and CYGB displayed no apparent methylation, while a combinatory epigenotype based on p16, hTERT, RASSF1 and WT1 was associated with a significantly higher chance of detecting malignancy in any positive sample (odds ratio: 24, 95% CI: 4.7-125, P<0.001). CONCLUSIONS: we demonstrate the successful application of qMSP to a large series of historical Ca ex PSA samples and report on a panel of TSGs with significant differences in their methylation profiles between benign and malignant variants of pleomorphic salivary adenoma. qMSP analysis could be developed as a useful clinical tool to differentiate between Ca ex PSA and its benign precursor.

| S-EPMC3008600 | biostudies-literature

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