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CircRNA sequencing analysis of PTMC with lateral lymph node metastases and related clinical value studies of circRNA.

ABSTRACT: CircRNA sequencing analysis of PTMC with lateral lymph node metastases and related clinical value studies of circRNA.

PROVIDER: PRJNA847254 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19589101_1.fastq.gz	Fastqsanger.gz
	SRR19589101_2.fastq.gz	Fastqsanger.gz
	SRR19589102_1.fastq.gz	Fastqsanger.gz
	SRR19589102_2.fastq.gz	Fastqsanger.gz
	SRR19589103_1.fastq.gz	Fastqsanger.gz

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CircRNA sequencing analysis of PTMC with lateral lymph node metastases and related clinical value studies of circRNA.

Project description:High-throughput sequencing was used to detect the expression profile of circRNA in PTMC of lymph node metastasis in lateral cervical region, and RT-qPCR was used to detect the expression of circRNA in cancer tissues and normal tissues adjacent to cancer. The receiver operating characteristic (ROC) curve and the area under ROC curve (AUC) were used to evaluate whether CircRNAs could be used as a biomarker for the diagnosis of thyroid cancer. Bioinformatics was used to predict the PTMC-related ceRNA mechanism of lateral cervical lymph node metastasis.

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Prognostic value of molecular intratumor heterogeneity in primary oral cancer and its lymph node metastases assessed by mass spectrometry imaging

Project description:Different aspects of intra-tumor heterogeneity (ITH), which are associated with development of cancer and its response to treatment, have postulated prognostic value. Here we searched for the potential association between phenotypic ITH analyzed by mass spectrometry imaging (MSI) and the prognosis of head and neck cancer. The study involved tissue specimens resected from 77 patients with locally advanced oral squamous cell carcinoma, including 37 patients where matched samples of primary tumor and synchronous lymph node metastases were analyzed. A 3-year follow-up was available for all patients that enabled their separation into two groups: with no evidence of disease (NED, n=41) and with progressive disease (PD, n=36). After the on-tissue trypsin digestion, peptide maps of all cancer regions were segmented using an unsupervised approach to reveal their intrinsic heterogeneity. We found that intra-tumor similarity of spectra was higher in the PD group and diversity of clusters identified during image segmentation was higher in the NED group, which indicated a higher level of ITH in patients with more favorable outcomes. Furthermore, signature of molecular components that correlated with long-term outcomes could be associated with proteins involved in the immune functions. Hence, we proposed that a higher level of ITH revealed by MSI in cancers with a better prognosis could reflect the presence of heterotypic components of tumor microenvironment such as infiltrating immune cells enhancing the response to the treatment. LC-MALDI-MS/MS data deposited in this repository originate from protein lysates obtained from FFPE sections consecutive to sections dedicated to MSI measurements. The hypothetical identity of the MSI components was established by assignment of a component location on the m/z scale for the measured masses of tryptic peptides identified by LC-MALDI-MS/MS allowing ±0.05% mass tolerance.

2022-10-13 | PXD034958 | Pride

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Project description:We performed oligonucleotide microarray analysis to assess the genetic expression alteration wich affected on lateral neck node metastasis of thyroid papillary microcarcinoma(PTMC). We performed microarray analysis in three PTMCs without cervical lymph-node metastases (N0), and five PTMCs with lateral neck-node metastasis (N1b) at initial diagnosis, using an Illumina HumanHT-12 v4.0 Expression BeadChip.

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