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Apilactobacillus kunkeei releases RNA-associated membrane vesicles and proteinaceous nanoparticles

ABSTRACT: Apilactobacillus kunkeei releases RNA-associated membrane vesicles and proteinaceous nanoparticles

PROVIDER: PRJNA848781 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19636011_1.fastq.gz	Fastqsanger.gz
	SRR19636011_2.fastq.gz	Fastqsanger.gz
	SRR19636012_1.fastq.gz	Fastqsanger.gz
	SRR19636012_2.fastq.gz	Fastqsanger.gz
	SRR19636013_1.fastq.gz	Fastqsanger.gz

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Apilactobacillus kunkeei releases RNA-associated membrane vesicles and proteinaceous nanoparticles

Project description:Extracellularly released particles, including membrane vesicles, have increasingly been recognized as important for bacterial community functions and host-interaction processes, but their compositions and functional roles differ between species and also between strains of the same species. In this study, we have determined the composition of membrane vesicles and protein particles identified in the cell-free pellets of two strains of Apilactobacillus kunkeei, a defensive symbiont of honeybees. The membrane vesicles were separated from the extracellular particles using density gradient ultracentrifugation. The peaks of the RNA and protein distributions were separated from each other and the highest concentration of RNA was observed in the fractions that contained the membrane vesicles while the highest protein concentration coincided with the fractions that contained extracellular particles. A comparative proteomics analysis by LC-MS/MS showed that 37 proteins with type-I signal peptides were consistently identified across the fractionated samples obtained from the cell-free pellets, of which 29 were orthologs detected in both strains. Functional predictions of the extracellular proteins revealed the presence of glycoside hydrolases, glycosyltransferases, giant proteins and peptidases. The extracellular transcriptomes mapped to a broad set of genes with a similar functional profile as the whole cell transcriptome. This study provides insights into the composition of membrane vesicles and extracellular proteins of a bee-associated symbiont.

2023-06-09 | GSE205998 | GEO

<i>Apilactobacillus kunkeei</i> releases RNA-associated membrane vesicles and proteinaceous nanoparticles.

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Characterization and composition of membrane vesicles and secreted proteins in Apilactobacillus kunkeei

Project description:Secreted particles, including membrane vesicles, have increasingly been recognized as important for bacterial community functions and host-interaction processes, but their specific compositions and functional roles are debated. In this study, we have characterized the secreted particles of Apilactobacillus kunkeei, a defensive symbiont of honeybees. We cultivated A. kunkeei strains A1401 and A0901 and separated the secreted protein particles from the extracellular membrane vesicles using density gradient ultracentrifugation. A proteomics analyses identified more than 500 proteins in each strain, of which 27 to 45 proteins were relatively more abundant in the cell-free supernatant, including glycoside hydrolases and peptidases. The extracellular transcriptome associated with the membrane vesicles contained a relatively higher fraction of mRNAs derived from highly transcribed operons such as those for ribosomal proteins and ATP synthase subunits, whereas highly expressed tRNAs were relatively more abundant in the cellular fraction. Based on these results, we propose that mRNAs for highly expressed proteins are overproduced and that superfluous mRNAs are fragmented, packaged into membrane vesicles and secreted. The results have implications for the utilization of membrane vesicles in A. kunkeei as a delivery tool for small RNA molecules, while also providing more general insights into the role of membrane vesicles in bacteria.

2023-09-07 | PXD034473 | Pride

Characterization and composition of membrane vesicles and secreted proteins in Apilactobacillus kunkeei

2023-09-07 | PXD034474 | Pride

Apilactobacillus kunkeei EFB6

Project description:Apilactobacillus kunkeei EFB6 Genome sequencing and assembly

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Apilactobacillus kunkeei strain:GL2

Project description:Apilactobacillus kunkeei strain:GL2 Genome sequencing

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Apilactobacillus kunkeei strain:CA1

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