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ISL1 controls pancreatic alpha cell fate and regulates beta cell differentiation and maturation [CUT&Tag]

ABSTRACT: ISL1 controls pancreatic alpha cell fate and regulates beta cell differentiation and maturation [CUT&Tag]

PROVIDER: PRJNA849186 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19649769_1.fastq.gz	Fastqsanger.gz
	SRR19649769_2.fastq.gz	Fastqsanger.gz
	SRR19649770_1.fastq.gz	Fastqsanger.gz
	SRR19649770_2.fastq.gz	Fastqsanger.gz
	SRR19649771_1.fastq.gz	Fastqsanger.gz

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ISL1 controls pancreatic alpha cell fate and regulates beta cell differentiation and maturation [CUT&Tag]

Project description:Glucose homeostasis is dependent on functional pancreatic α and ß cells. Mechanisms underlying generation and maturation of these endocrine cells remain unclear. Here, we unravel the molecular mode of action of ISL1 in controlling α cell fate and endocrine differentiation in the pancreas. By combining transgenic mouse models, transcriptomic and epigenomic profiling, we uncover that elimination of Isl1 results in a diabetic phenotype with a complete loss of α cells, disrupted pancreatic islet architecture, downregulation of maturation markers of ß cells, and an enrichment in an intermediate endocrine progenitor transcriptomic profile. Mechanistically, apart from the altered transcriptome of pancreatic endocrine cells, Isl1 elimination results in altered silencing H3K27me3 histone modifications in the promoter regions of the essential genes for endocrine cell differentiation. Our results thus show that ISL1 transcriptionally and epigenetically controls α cell fate competence, and ß cell maturation, suggesting that ISL1 is a critical component for generating functional α and ß cells.

2022-06-17 | GSE206092 | GEO

ISL1 controls pancreatic alpha cell fate and regulates beta cell differentiation and maturation [RNA-seq]

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Isl1 identifies the extraembryonic mesodermal / allantois progenitors and required for placenta morphogenesis and vasculature formation [CUT&Tag]

Project description:To uncover direct targets of ISL1 that could account for its role in allantois, we performed cut tag analysis on wild type allantoic cells using ISL1 antibody at E8.75. Our analyses uncovered 5509 significant binding peaks for ISL1, mainly located at intergenic or intronic regions.

2024-09-13 | GSE247183 | GEO

ISL1 controls pancreatic alpha cell fate and regulates beta cell differentiation and maturation

Project description:ISL1 controls pancreatic alpha cell fate and regulates beta cell differentiation and maturation

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DOT1L controls NK cell maturation and lineage integrity [CUT&TAG]

Project description:DOT1L controls NK cell maturation and lineage integrity [CUT&TAG]

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ISL1 controls pancreatic alpha cell fate and regulates beta cell differentiation and maturation [RNA-seq]

Project description:ISL1 controls pancreatic alpha cell fate and regulates beta cell differentiation and maturation [RNA-seq]

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ISL1 and POU4F1 Directly Interact to Regulate the Differentiation and Survival of Inner Ear Sensory Neurons

Project description:Isl1 and Pou4f1 regulate a unique set of CVG gene cooperatively or individually. GO term analysis shows these genes are crucial for CVGs development. To estimate Isl1 and Pou4f1 directly or indirectly regulate these downstream genes and how they act to affect these genes, we performed the CUT&Tag of ISL1 and POU4F1 with the histone modifications, H3K4me3, H3K27ac and H3K27me3 in CVGs. The identity of the peaks were characterized and big chromatin profiles were obtained.

2024-05-31 | GSE233840 | GEO

scNanoSeq-CUT&Tag: a long-read single-cell CUT&Tag sequencing method for efficient chromatin modification profiling within individual cells

Project description:We developed scNanoSeq-CUT&Tag, a streamlined method by adapting a modified CUT&Tag protocol to Oxford Nanopore sequencing platform for efficient chromatin modification profiling at single-cell resolution. We firstly tested the performance of scNanoSeq-CUT&Tag on six human cell lines: K562, 293T, GM12878, HG002, H9, HFF1 and adult mouse blood cells, it showed that scNanoSeq-CUT&Tag can accurately distinguish different cell types in vitro and in vivo. Moreover, scNanoSeq-CUT&Tag enables to effectively map the allele-specific epigenomic modifications in the human genome andallows to analyze co-occupancy of histone modifications. Taking advantage of long-read sequencing,scNanoSeq-CUT&Tag can sensitively detect epigenomic state of repetitive elements. In addition, by applying scNanoSeq-CUT&Tag to testicular cells of adult mouse B6D2F1, we demonstrated that scNanoSeq-CUT&Tag maps dynamic epigenetic state changes during mouse spermatogenesis. Finally, we exploited the epigenetic changes of human leukemia cell line K562 during DNA demethylation, it showed that NanoSeq-CUT&Tag can capture H3K27ac signals changes along DNA demethylation. Overall, we prove that scNanoSeq-CUT&Tag is a valuable tool for efficiently probing chromatin state changes within individual cells.

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