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ABSTRACT: Transcriptome data of mouse peritoneal macrophages

PROVIDER: PRJNA849350 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28904177_1.fastq.gz	Fastqsanger.gz
	SRR28904177_2.fastq.gz	Fastqsanger.gz
	SRR28904178_1.fastq.gz	Fastqsanger.gz
	SRR28904178_2.fastq.gz	Fastqsanger.gz
	SRR28904179_1.fastq.gz	Fastqsanger.gz

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Gene expression profile (RNAseq) of wild-type and Zeb1 (+/-) peritoneal mouse macrophages

Project description:Purpose: Characterize the gene expression profile of wild-type and Zeb1 (+/-) peritoneal mouse macrophages through RNA sequencing Methods: RNA sequencing of RNA from peritoneal macrophages isolated by peritoneal lavage and FACS sorting (F4/80+ CD11b+) from wild-type and Zeb1 (+/-) mice (2 mice pooled for each sample/set) Results: Wild-type and Zeb1 (+/-) peritoneal mouse macrophages display differential gene expression. Zeb1 (+/-) peritoneal macrophages express lower levels of SPM (F4/80low)-associated genes Conclusions: Wild-type and Zeb1 (+/-) peritoneal mouse macrophages display differential gene expression. Zeb1 (+/-) peritoneal macrophages express lower levels of SPM (F4/80low)-associated genes

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Project description:Purpose: Characterize the gene expression profile of of peritoneal mouse macrophages in Endotoxic shock and Tolerance through RNA sequencing Methods: RNA sequencing of RNA from peritoneal macrophages in Endotoxic shock and Tolerance isolated by peritoneal lavage and FACS sorting (F4/80+ CD11b+) Results: Endotoxic shock and Tolerance peritoneal mouse macrophages display differential gene expression. Conclusions: Endotoxic shock and Tolerance peritoneal mouse macrophages display differential gene expression.

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Project description:Peritoneal macrophages transcriptome

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Project description:RNA transcriptome data from C57BL/6 tissue resident peritoneal macrophages over expressing microRNA 708 or control. The role of microRNA-708 in shaping macrophage biology remains mostly unknown. Here, using lentiviral vectors we overexpressed microRNA-708 in vivo in C57BL/6 mice peritoneal macrophages and investigated mRNA changes in these cells after 4 days.

2024-05-03 | E-MTAB-13782 | biostudies-arrayexpress

Project description:Sequencing of mouse peritoneal macrophages.

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Project description:WGBS data from peritoneal macrophages of C57BL/6 mouse

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Mus musculus

Project description:Expression data from peritoneal macrophages

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Next-Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Kdm2b-/- Peritoneal Macrophage Transcriptomes

Project description:Purpose: The purpose of this study is to detect activated or silenced genes during Kdm2b-deficient peritoneal macrophages and the control cells. Gene expression differences between two samples could be found using transcriptome profiling (RNA-seq) analysis. Methods: Mouse peritoneal macrophages were harvested from mice 4 days after thioglycollate (BD, Sparks, MD) injection. Macrophages RNA profiles were generated by deep sequencing, using Illumina. Results: We mapped about 10 million sequence reads per sample to the mouse genome, identified hundreds of genes with significant mRNA variation between Kdm2b-deficient macrophages and the control cells.

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