Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of knockdown of DIO3OS and PTBP1 on gene expression of MCF-7 LTED cells [cellseq]

ABSTRACT: Effect of knockdown of DIO3OS and PTBP1 on gene expression of MCF-7 LTED cells [cellseq]

PROVIDER: PRJNA849412 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Effect of knockdown of DIO3OS and PTBP1 on gene expression of MCF-7 LTED cells [cellseq]

Project description:To investigate the function of DIO3OS and PTBP1 in the metabolic regulation, we used LNA and siRNA to silence DIO3OS and PTBP1, respectively, in established MCF-7 LTED cell lines. We then performed gene expression profiling analysis using data obtained from RNA-seq of six different cells.

2022-10-11 | GSE206142 | GEO

Effect of estradiol (E2) exposure and KMT2B knockdown on gene expression in MCF-7 cells

Project description:MCF-7 is an estrogen receptor-positive breast cancer cell line. This experiment is designed to study (1) the effect of estradiol (E2) exposure and (2) lysine methyltransferase 2B (KMT2B) knockdown in MCF-7 cells. Cells were grown for 72 hours prior to treatment with vehicle or 10 nM E2 for 4 and 24 hours. Additionally, to assess the effect of KMT2B knockdown, MCF-7 cells were transfected with KMT2B targeting siRNA or scrambled control siRNA in the absence or presence of E2. RNA were isolated using Trizol and hybridized to Affymetrix GeneChip Human Genome U133 Plus 2.0 array.

2017-01-30 | E-MTAB-4923 | biostudies-arrayexpress

Transcriptomic analysis of senescent cells upon PTBP1 knockdown.

Project description:IMR90 ER:RAS cells were stably transduced with either an empty vector or 2 deconvoluted shRNAs targeting PTBP1. Following selection with puromycin, the cells were treated with 4OHT to induce senescence. 6 days later the cells were collected for total mRNA analysis. PTBP1 is a regulator of alternative splicing. Our previous experiments had shown that PTBP1 depletion inhibits the expression of pro-inflammatory genes without affecting other senescence-associated phenotypes. By performing RNA-seq we confirmed those observations at a global level and analysed how PTBP1 knockdown alters alternative splicing as a potential mechanism of action.

2018-06-25 | GSE101750 | GEO

The effect of CBX2 knockdown on gene expression in MCF-7 cells

Project description:The effect of CBX2 knockdown on gene expression in MCF-7 cells

| PRJNA815062 | ENA

Effect of degradation of IGFRIL/PTBP1 in HepG2 cells

Project description:To investigate the effect of IGFRIL/PTBP1 in HCC, we established HepG2 cell lines in which IGFRIL/PTBP1 mRNA has been degraded using siRNA.

2023-05-13 | GSE232354 | GEO

RNA Sequencing Facilitates Quantitative Analysis of differentially expressed genes after PTBP1 knockdown in human erythroid cells

Project description:Purpose: The goals of this study are to determine possible downstream targets of PTBP1 on day8 of erythroid differentiation with or without PTBP1 knockdown. Methods:gene expression profiling with or without PTBP1 knockdown in differentiated erythroblasts at day8 were generated by deep sequencing, induplicate, using Illumina GAIIx.The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using TaqMan and SYBR Green assays. Conclusions: heme metabolism genes were down-regulated after PTBP1 knockdown during erythroid differentiation

2018-10-30 | GSE106566 | GEO

Altered expression of genes in lncRNA-LBCS, hnRNPK and PTBP1 knockdown

Project description:We identify a lncRNA which low expresses and regulates self-renewal of bladder cancer stem cell. So we named it lncRNA-LBCS (low expresses in bladder cancer stem cell). Further study finds that lncRNA-LBCS bind to hnRNPK and PTBP1. To investigate the genes regulated by these, we knockdown lncRNA-LBCS, hnRNPK and PTBP1 by siRNA, respectively, and perform high-throughput sequence. So we identify a series of genes regulated by lncRNA-LBCS, hnRNPK and PTBP1, respectively.

2017-08-13 | GSE79832 | GEO

Effect of SETD1A knockdown on global gene expression in MCF-7 cells

Project description:SETD1A is a histone H3K4 methyltransferase and function as a coactivator for nuclear receptors (NRs) and other transcription factors. We performed genome-wide gene expression analysis in non-specific siRNA transfected or SETD1A knockdown MCF-7 cells to investigate global gene expression changes induced by SETD1A knockdown.

2018-10-24 | GSE101860 | GEO

Transcriptomic analysis of senescent cells upon PTBP1 knockdown and EXOC7 knockdown

Project description:Transcriptomic analysis of senescent cells upon PTBP1 knockdown and EXOC7 knockdown

| PRJNA395374 | ENA

Expression analysis upon SIRT1 knockdown in MCF-7 cells

Project description:SIRT1 is a nuclear NAD+-dependent protein deacetylase. Expression microarray analysis was used to study the effect of SIRT1 knockdown on gene expression in MCF-7 breast cancer cells.

2009-05-28 | GSE13459 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data