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Effect of knockdown of DIO3OS and PTBP1 on gene expression of MCF-7 LTED cells [cellseq]

ABSTRACT: Effect of knockdown of DIO3OS and PTBP1 on gene expression of MCF-7 LTED cells [cellseq]

PROVIDER: PRJNA849412 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19660767_1.fastq.gz	Fastqsanger.gz
	SRR19660767_2.fastq.gz	Fastqsanger.gz
	SRR19660768_1.fastq.gz	Fastqsanger.gz
	SRR19660768_2.fastq.gz	Fastqsanger.gz
	SRR19660769_1.fastq.gz	Fastqsanger.gz

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Effect of knockdown of DIO3OS and PTBP1 on gene expression of MCF-7 LTED cells [cellseq]

Project description:To investigate the function of DIO3OS and PTBP1 in the metabolic regulation, we used LNA and siRNA to silence DIO3OS and PTBP1, respectively, in established MCF-7 LTED cell lines. We then performed gene expression profiling analysis using data obtained from RNA-seq of six different cells.

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Project description:Purpose: The goals of this study are to determine possible downstream targets of PTBP1 on day8 of erythroid differentiation with or without PTBP1 knockdown. Methods:gene expression profiling with or without PTBP1 knockdown in differentiated erythroblasts at day8 were generated by deep sequencing, induplicate, using Illumina GAIIx.The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using TaqMan and SYBR Green assays. Conclusions: heme metabolism genes were down-regulated after PTBP1 knockdown during erythroid differentiation

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