Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The evolutionarily conserved gene Arrdc5 is required for male fertility in mice

ABSTRACT: The evolutionarily conserved gene Arrdc5 is required for male fertility in mice

PROVIDER: PRJNA849507 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR19661979.fastq.gz	Fastqsanger.gz
	SRR19661980.fastq.gz	Fastqsanger.gz
	SRR19661981.fastq.gz	Fastqsanger.gz
	SRR19661982.fastq.gz	Fastqsanger.gz
	SRR19661983.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

The evolutionarily conserved gene Arrdc5 is required for male fertility in mice

Project description: In sexually reproducing organisms, sperm contribute half the genomic material required for creation of offspring and natural fertility relies on males producing millions by way of spermatogenesis. This process is essential for continuity and diversity of a species, yet evolutionarily conserved core molecular regulators are undefined. Here we used single cell RNA-sequencing of testicular tissue from mice, pigs, and cattle to generate a multispecies integrated transcriptome database. Through bioinformatic analysis, the gene arrestin-domain containing 5 (Arrdc5) which encodes for an α-arrestin molecule was identified as a potential evolutionarily conserved regulator of spermatogenesis. Expression of Arrdc5 is testis specific in mice, pigs, cattle, and humans. Knockout of Arrdc5 in mice leads to male specific sterility due to production of low numbers of sperm that are immotile and malformed, resembling oligoasthenoteratospermia (OAT) which is a common diagnosis of infertility in men. Spermiogenesis, the final phase of spermatogenesis when round spermatids morphologically transform to spermatozoa, is defective in testes of Arrdc5 deficient mice. Also, epididymal sperm in Arrdc5 knockouts are unable to capacitate and fertilize oocytes. These findings establish Arrdc5 as a core regulator of mammalian spermatogenesis. Considering the role of arrestin molecules as modulators of G-protein coupled signaling and ubiquitination, Arrdc5 is a potential male contraceptive target.

2023-03-06 | GSE206156 | GEO

NAT10-mediated N4-Acetylcytidine is Required for Spermatogenesis and Male Fertility

Project description:NAT10-mediated N4-Acetylcytidine is Required for Spermatogenesis and Male Fertility

| PRJNA789775 | ENA

Tsga8 is required for spermatid morphogenesis and male fertility in mice

Project description:Tsga8 is required for spermatid morphogenesis and male fertility in mice

| PRJNA619065 | ENA

The evolutionarily conserved piRNA-producing locus pi6 is required for male mouse fertility.

Project description:Pachytene PIWI-interacting RNAs (piRNAs), which comprise >80% of small RNAs in the adult mouse testis, have been proposed to bind and regulate target RNAs like microRNAs, cleave targets like short interfering RNAs or lack biological function altogether. Although piRNA pathway protein mutants are male sterile, no biological function has been identified for any mammalian piRNA-producing locus. Here, we report that males lacking piRNAs from a conserved mouse pachytene piRNA locus on chromosome 6 (pi6) produce sperm with defects in capacitation and egg fertilization. Moreover, heterozygous embryos sired by pi6-/- fathers show reduced viability in utero. Molecular analyses suggest that pi6 piRNAs repress gene expression by cleaving messenger RNAs encoding proteins required for sperm function. pi6 also participates in a network of piRNA-piRNA precursor interactions that initiate piRNA production from a second piRNA locus on chromosome 10, as well as pi6 itself. Our data establish a direct role for pachytene piRNAs in spermiogenesis and embryo viability.

| S-EPMC7383350 | biostudies-literature

Mus musculus

Project description:An Evolutionarily Conserved piRNA-producing Locus Required for Male Mouse Fertility

| PRJNA480354 | ENA

RNF20 is required for male fertility through H2B ubiquitination in Sertoli cells

Project description:RNF20 is required for male fertility through H2B ubiquitination in Sertoli cells

| PRJNA934381 | ENA

Impact of anti-tumor necrosis factors antibodies treatment on fertility of inflammatory bowel diseases male patients

Project description:Primary objectives: To assess the impact of antiTNF agents on the male inflammatory bowel diseases patients` fertility Primary outcomes:- semen quality and quantity, inclusive sperm chromatine structure analysis prior and during anti-TNF therapy- time to conception

| 2525194 | ecrin-mdr-crc

Mus musculus strain:C57BL/6

Project description:The Evolutionarily Conserved piRNA Producing Locus pi6 Is Required for Male Mouse Fertility

| PRJNA634688 | ENA

A transphyletic approach to analyzing evolutionarily conserved β-catenin modules

Project description:To identify evolutionarily conserved Beta-catenin protein interactions, Beta-catenin mRNA from various metazoans was injected into Xenopus embryos and immunopurified at gastrula stage. Beta-catenin complexes were then separated on an SDS-PAGE gel and subjected mass spectrometric analysis

2023-07-08 | PXD035167 | JPOST Repository

GIV/Girdin Regulates Spatiotemporal Signaling during Sperm Capacitation and is Required for Male Fertility

Project description:GIV/Girdin Regulates Spatiotemporal Signaling during Sperm Capacitation and is Required for Male Fertility

| PRJNA720623 | ENA