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Mus musculus strain:mouse embryonic fibroblast (MEF) cells line NIH3T3

ABSTRACT: Mus musculus strain:mouse embryonic fibroblast (MEF) cells line NIH3T3 Raw sequence reads

PROVIDER: PRJNA849633 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19666921_1.fastq.gz	Fastqsanger.gz
	SRR19666921_2.fastq.gz	Fastqsanger.gz
	SRR19666922_1.fastq.gz	Fastqsanger.gz
	SRR19666922_2.fastq.gz	Fastqsanger.gz
	SRR19666923_1.fastq.gz	Fastqsanger.gz

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Mus musculus strain:mouse embryonic fibroblast (MEF) cells line NIH3T3

Project description:Mus musculus strain:mouse embryonic fibroblast (MEF) cells line NIH3T3 Raw sequence reads

| PRJNA765330 | ENA

Gene expression analysis of inositol hexakisphosphate kinase 1 (IP6K1) knockout mouse embryonic fibroblasts

Project description:To investigate the biological significance of IP6K1 function we compared the gene expression profile of two mouse embryonic fibroblast (MEF) cell lines each derived from Ip6k1+/+ and Ip6k1-/- embryos. Results reveal downregulation of several biological processes upon the loss of IP6K1. Organism : Mus musculus , Agilent Gene Expression Whole Genome Mouse 4x44k (AMADID: 14868). Two biological replicates each of Ip6k1+/+ and Ip6k1-/- MEFs

2016-05-16 | E-GEOD-73612 | biostudies-arrayexpress

Gene expression in gamma-glutamylcyclotransferase-overexpressing mouse embryonic fibroblast NIH3T3 cells.

Project description:Gene expression in gamma-glutamylcyclotransferase-overexpressing mouse embryonic fibroblast NIH3T3 cells.

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Analysis of the transcriptomic profile of WT mouse embryonic fibroblast (MEF WT) and Nf2-knockout mouse embryonic fibroblast (MEF Nf2 KO) cell lines

Project description:Analysis of the transcriptomic profile of WT mouse embryonic fibroblast (MEF WT) and Nf2-knockout mouse embryonic fibroblast (MEF Nf2 KO) cell lines

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Role of SIRT7 overexpression in mouse fibroblast cells NIH3T3

Project description:Role of SIRT7 overexpression in mouse fibroblast cells NIH3T3

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Role of SIRT7 overexpression in mouse fibroblast cells NIH3T3

Project description:Human SIRT7 GFP or GFP alone were overexpressed in NIH3T3 cells.In confluent conditions, SIRT7GFP overexpressing cells but not GFP expressing cells showed presence of distinct compact cellular colonies composed of small sized cells (ST7). Along with this SIRT7GFP overexpressing cells with long and fibroblast morphoogy(LT7) were also present. Single cell clones from these cells were expanded to form ST7 (Small SIRT7GFP expressing) and LT7 (Long SIRT7GFP expressing) lines. RNA from GFP expressing cells (SKGFP), ST7 and LT7 lines was then isolated and applied to microarray analysis to identify gene expression changes in two cell lines

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Gene expression in gamma-glutamylcyclotransferase-overexpressing mouse embryonic fibroblast NIH3T3 cells.

Project description:Although hypoxia inducible factor-1a (HIF-1a) was originally identified as a transcriptional factor to adapt hypoxia, HIF-1a has also been shown to regulate metabolic pathways specific for cancer, including aerobic glycolysis, namely “Warburg effect”. However, the mechanisms by which HIF-1a mediates the metabolic pathway under normoxia are not fully understood. Here, we identified gamma-glutamylcyclotransferase (GGCT) as a novel regulator of HIF-1a. GGCT is a highly expressed protein in various cancer tissues. Knockdown of GGCT exerts anticancer effects both in vitro and in vivo; thus, it is considered a promising therapeutic target. In this study, we show that depleting GGCT downregulates the protein and mRNA expressions of HIF-1a in PC3 prostate cancer cells, and that overexpressing GGCT upregulates them in mouse fibroblast NIH3T3 cells. We also show that depleting GGCT downregulates HIF-1a downstream target genes involved in glycolysis, whereas overexpressing GGCT exhibits upregulation pattern in these genes. Furthermore, we suggest that GGCT induces the metabolic switch from citric acid cycle to glycolysis under normoxia. Additionally, we show that GGCT regulates expression of HIF-1a protein via AMPK-mTORC1-4EBP1 pathway in PC3 cells. These results indicate the GGCT plays a regulatory role in the expression of HIF-1a followed by glycolytic switch in cancer cells.

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polyA mRNA RNA-seq from embryonic fibroblast (ENCSR000CGV)

Project description:RNA-seq on mouse embryonic fibroblast (MEF) For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2017-02-03 | GSE93454 | GEO

CTCF binding profiles in MEF cells

Project description:We sequenced CTCF binding sites in Akt1+/+ or Akt1-/- mouse embryonic fibroblast (MEF) cell line.

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Identification of Protein Expression Changes During Fibroblast Activation in Response to LAMP2A Overexpression

Project description:Proteomic analysis comparing LAMP2A-TGFβtreated group versus Vector-TGFβ 3 treated group in NIH3T3 fibroblast cells to study the effect of LAMP2A overexpression on protein expression changes During Fibroblast Activation.

2024-09-02 | PXD055463 |

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