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ABSTRACT: Ipomoea batatas (sweet potato)

PROVIDER: PRJNA849767 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19668063_1.fastq.gz	Fastqsanger.gz
	SRR19668063_2.fastq.gz	Fastqsanger.gz
	SRR19668064_1.fastq.gz	Fastqsanger.gz
	SRR19668064_2.fastq.gz	Fastqsanger.gz
	SRR19668065_1.fastq.gz	Fastqsanger.gz

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Gene expression after SPVD infection in resistant and susceptible sweet potato cultivars

Project description:Sweet potato virus disease (SPVD) is one of the most devastating diseases affecting sweetpotato (Ipomoea batatas), an important food crop in developing countries. SPVD develops when sweetpotato plants are dually infected with sweet potato feathery mottle virus (SPFMV) and sweet potato chlorotic stunt virus (SPCSV). In the current study, global gene expression between SPVD affected plants and virus-tested control plants (VT) were compared in the susceptible ‘Beauregard’ and resistant ‘NASPOT 1’ (Nas) sweetpotato cultivars at 5, 9, 13 and 17 days post inoculation (DPI).

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Analysis of global gene expression signatures in sweet potato as affected by temperature stress

Project description:Global gene expression signatures was analysed through microarray expression profiling as a discovery platform to identify up and down regulated ESTs that represent genes involved in metabolic pathways in the leaf, fibrous root and storage root (tuber forming root) of sweetpotato (Ipomoea batatas) as affcted by high temperature stress (40oC) compared to ambient temperature (30oC). Also Global gene expression signatures was analysed by the same procedure to explore up and down regulated ESTs in tuberous root of sweet potato in comparison with fibrous root of Ipomoea cornea and identify unique ESTs that represent genes involved in tuber formation in sweet potato.

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Project description:Sweet potato (Ipomoea batatas), a starchy root and tuber crop, is an important source of carbohydrates for the human diet and a valuable resource in many industrial applications. Here, we performed a spatiotemporally distributed proteome analysis using leaves and storage roots of two F1 individuals, H283 (high starch) and L423 (low starch), of similar high yield to underly regulatory networks governing starch accumulation.

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Project description:To screen genes related to the development of sweet potato tuberous roots, the high throughput sequencing of different stages of sweet potato tuberous roots was performed. The fibrous roots (FR; roots at 20 dap), developing tuberous roots (DR; roots at 60 dap) and mature tuberous roots (MR; roots at 120 dap) of Ipomoea batatas (L.) Taizhong 6 and MBP3 overexpressed lines were used for transcriptome analysis. Totally, we identified 5488 differentially expressed genes between different stage tuberous roots of Taizhong6 and 14312 differentially expressed genes between the tuberous roots of Taizhong6 and MBP3 overexpressed lines, by calculating the gene FPKM in each sample and conducting differential gene analysis. This study provides a foundation for the mechanism analysis of sweet potato tuberous root development.

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Temporal patterns of gene expression associated with tuberous root formation and development in sweetpotato (Ipomoea batatas)

Project description:Investigation of whole transcriptome gene expression level during tuberous root formation and development in sweetpotato (Ipomoea batatas) cv. Guangshu 87 Identification of transcription factors (TFs) during tuberous root formation and development in sweetpotato (Ipomoea batatas) cv. Guangshu 87 A total of 7 samples were analyzed using RNA isolated from sweetpotato roots at 10, 15, 20, 30, 60, 90, 120days after transplanting. Each sample had two biological replicates.

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Project description:Identification of a DAMP receptor and its cognate peptide ligand in sweet potato (Ipomoea batatas)

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