Project description:Axolotl limb regeneration proceeds through the formation of a blastema, a mound of progenitor cells that accumulate at the end of the amputated stump. These progenitor cells expand and later undergo patterning to regenerate the missing limb, restoring both form and function. A subset of cells within the blastema become senescent, a state of permanent growth arrest. Here, we address the functional relevance of cellular senescence to axolotl limb regeneration, through a combination of gain- and loss-of-function assays. Using transcriptomic analyses on in vitro and in vivo senescent cells, we gain insights into the basis of the senescent phenotype, cell-cycle arrest, and molecular mediators involved in axolotl regeneration at the molecular level.
Project description:In order to identify the transcriptome change and heterogeneity in replicative senescent cells and stress-induced senescent cells, =we measured 1600 single cell transcriptomes of young quiescent cells at a population doubling (PD) number of 38, middle age quiescent cells (PD = 48), replicative senescent (PD = 71) cells and 50Gy X-ray induced senescent cells of PD38 control cells by Drop-seq.
Project description:We report the analysis of single cell based sequencing to understand the cellular and molecular heterogeneity of senescent cell populations in vivo in multiple organ types. The findings will help characterise the roles different cell types play during the aging process.
Project description:We report the analysis of single cell based sequencing to understand the cellular and molecular heterogeneity of senescent cell populations in vivo in multiple organ types. The findings will help characterise the roles different cell types play during the aging process.
Project description:We report the analysis of single cell based sequencing to understand the cellular and molecular heterogeneity of senescent cell populations in vivo in multiple organ types. The findings will help characterise the roles different cell types play during the aging process.
Project description:There is an urgent need to comprehensively catalog senescence markers across the wide range of cell types in an organism. Here, we profiled the transcriptomes and proteomes of over 30 models of senescence in 14 different primary human cell types. We found that senescent cells from all tissue types do not share a unique marker, but they do share broadly activated or repressed pathways, namely pathways of damage response to elicit tissue repair, and unique metabolic pathways. Importantly, the combined use of some of the most widely shared senescence markers validated the presence of senescent-like cells in mice through single-cell RNA-sequencing and immunostaining approaches. The enclosed catalog represents a much-needed resource to identify senescent cells across tissues in the body.