Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: MDR Acinetobacter baumannii

PROVIDER: PRJNA850903 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR19751061_1.fastq.gz	Fastqsanger.gz
	SRR19751061_2.fastq.gz	Fastqsanger.gz
	SRR19751062_1.fastq.gz	Fastqsanger.gz
	SRR19751062_2.fastq.gz	Fastqsanger.gz
	SRR19751063_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 62

Similar Datasets

Combined analysis of genome-wide DNA methylome and transcriptome reveals the first epigenetic-based antibiotic-resistance mechanism in Acinetobacter baumannii

Project description:Using Nanopore sequencing, our study has revealed a close correlation between genomic methylation levels and antibiotic resistance rates in Acinetobacter Baumannii. Specifically, the combined genome-wide DNA methylome and transcriptome analysis revealed the first epigenetic-based antibiotic-resistance mechanism in A. baumannii. Our findings suggest that the precise location of methylation sites along the chromosome could provide new diagnostic markers and drug targets to improve the management of multidrug-resistant A. baumannii infections.

2023-09-01 | E-MTAB-13272 | biostudies-arrayexpress

3D-printed wound dressings containing a fosmidomycin-derivative prevent Acinetobacter baumannii biofilm formation in vitro and in vivo

Project description:Two Acinetobacter baumannii strains with low susceptibility to fosmidomycin and two reference with high susceptibility to fosmidomycin were DNA-sequenced to investigate the genomic determinants of fosmidomycin resistance.

2022-12-21 | E-MTAB-12357 | biostudies-arrayexpress

In vitro and In vivo Antimicrobial Potential of Lithium Complex against Multi Drug Resistant Acinetobacter baumannii

Project description:Acinetobacter baumannii is a Gram-negative ESKAPE pathogen, accountable for lethal nosocomial infections, owing to its high pathogenicity and multi-drug resistance (MDR). Hence, the World Health Organization (WHO) has prioritized this bacterium against which new pharmacophores are required urgently. Therefore, this study evaluates the antimicrobial potential of lithium complex based on salicylic acid, and 1,10-phenanthroline ([Li(phen)2sal]) against MDR A. baumannii (MDRAB) through growth inhibition, time-kill assay, biofilm inhibition, and eradication alongside microscopy. The mechanism of action was proposed by flow cytometry and proteomic analysis. Moreover, its in-vivo efficacy was assessed using A. baumannii-induced pneumonia model in mice. Results showed that lithium complex significantly inhibited the bacterial growth at 16-32 µg/ml. Time kill analysis revealed that the compound retarded the bacterial growth after 2-3 h of treatment. Lithium complex significantly inhibited and eradicated >70% of biofilms at the MIC level which is a key problem of indwelling and prosthetic devices. Light and atomic force microscopy further indicated disarticulated biofilm and ruptured cellular membrane post-lithium complex treatment. Proteomic analysis revealed that lithium complex exerted oxidative stress on A. baumannii which may contribute to cell rupturing. Flow cytometry confirmed the role of lithium complex in cytoplasmic membrane disintegration. The compound ameliorated the pneumonia symptoms at non-toxic doses of 20 and 40 mg/kg in the pre-clinical A. baumannii-induced pneumonia model as evident by the significant reduction in bacterial burden and parenchymal inflammation. Taken together, these findings suggest that the lithium complex can be a potential drug candidate against MDR A. baumannii.

2023-09-22 | PXD044293 | Pride

Transcription profiling of Acinetobacter baumannii multi-drug resistant strain and a susceptible reference strain - Abefflux

Project description:Transcriptomic comparison of a multidrug resistant Acinetobacter baumannii strain and a susceptible reference strain.

2009-11-20 | E-MEXP-2254 | biostudies-arrayexpress

Colistin resistant A. baumannii: genomic and transcriptomic traits acquired under colistin therapy

Project description:Comparative Genomic and Transcriptomic Characterization of Colistin-Resistant Acinetobacter baumannii

2019-01-23 | GSE109951 | GEO

Transcriptome profiling of wild type and ΔabaI::Km mutant

Project description:Transcriptome profiling of quorum sensing regulated genes in Acinetobacter baumannii 863

2019-06-21 | GSE120346 | GEO

Comparison of the gene expression profiles of different strains of A. baumannii by microarrays

Project description:In the present work we compare the gene expression profile of A. baumannii and a mutant knock-out strain of A. baumannii lacking a small RNA gene 13573 and the corresponding small RNA 13573 over-producing strain. The main objective is to recognize the main pathways in which the small RNA 13573 is involved. Moreover, the same wild type strain was used to infect mice and was further analyzed after the infection with the aim of finding genes differentially expressed in vivo. Three biological replicates have been performed for each comparison. The RNA collection from Acinetobacter baumannii strain over-expresing the small RNA (sample 13573) was compared with this isolated from A. baumannii harboring the empty vector (PETRA sample) while gene expression in the knock-out strain (KO sample) was compared with the wild type strain Acinetobacter baumannii ATCC 17978 (ATCC sample). The RNA from A.baumannii recovered from the infected animals (INF sample) was compared with the wild type (ATCC).

2016-08-01 | E-GEOD-60348 | biostudies-arrayexpress

RNA-seq of evolved strains from Acinetobacter baumannii NCCP 16007

Project description:Comparison for gene expression of Acinetobacter baumannii NCCP 16007 by evolved strains

2022-11-16 | GSE217840 | GEO

Transcriptomic profiling by RNA-seq of A. baumannii 17978 wild-type, and ∆A1S_1874 mutants.

Project description:Acinetobacter baumannii A1S_1874 gene encodes as a LysR-type transcriptional regulator. LysR family regulators known to regulate biofilm formation, antibiotic resistance, and the expression of diverse genes in other Gram-negative bacteria. However, A1S-1874 has never been characterized in Acinetobacter baumannii, and the studies about the regulon of A1S-1874 are not discovered. In this study we revealed that A1S_1874 differentially regulates at least 302 genes including the csu pilus operon, N-acylhomoserine lactone synthese gene, A1S_0112-A1S_0118 operon, type 1v secretion system related genes that are involved in biofilm formation, surface motility, adherence, quorum sensing and virulence. Overall, our data suggests that A1S-1874 is a key regulator of Acinetobacter baumannii biofilm formation and gene expression.

2021-05-25 | GSE173626 | GEO

RNA-seq of Acinetobacter baumannii ATCC 17978 Lab-WT: under blue light

Project description:Comparison for gene expression of Acinetobacter baumannii Lab-WT under blue light irradiation

2022-01-31 | GSE195643 | GEO