Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Bulk mRNA sequencing of adult opossum tissues

ABSTRACT: Bulk mRNA sequencing of adult opossum tissues

PROVIDER: PRJNA851077 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR19754600_1.fastq.gz	Fastqsanger.gz
	SRR19754600_2.fastq.gz	Fastqsanger.gz
	SRR19754601_1.fastq.gz	Fastqsanger.gz
	SRR19754601_2.fastq.gz	Fastqsanger.gz
	SRR19754602_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 114

Similar Datasets

Bulk mRNA sequencing of adult opossum tissues

Project description:Decades of work in placental (eutherian) species have constructed a paradigm of mammalian development, wherein the genome-wide erasure of parental DNA methylation is required for embryogenesis1-10. Whether such DNA methylation reprogramming is, in fact, conserved in other mammals is unknown. To resolve this point, we generated base-resolution DNA methylation maps in gametes, embryos and adult tissues of the opossum marsupial Monodelphis domestica, revealing extensive variations from the eutherian-derived model. In stark contrast with eutherians, the marsupial genome remains hypermethylated during the cleavage stages and in the embryo proper of the blastocyst. In the extra-embryonic trophectoderm DNA methylation is reduced, suggesting an important evolutionary conserved function for DNA hypomethylation in formation of the mammalian placenta. Furthermore, unlike in eutherians, the inactive X chromosome becomes globally DNA hypomethylated during embryogenesis. Using our DNA methylation profiles, we identify a candidate mechanism for imprinted X-inactivation in marsupials, via maternal promoter DNA methylation of the Xist-like non-coding RNA RSX11. How mammalian embryos employ DNA methylation to regulate their development is therefore more mechanistically diverse than current models can accommodate.

2025-01-12 | GSE206498 | GEO

Whole genome bisulfite sequencing of adult opossum tissues

Project description:Whole genome bisulfite sequencing of adult opossum tissues

| PRJNA850431 | ENA

Whole genome bisulfite sequencing of adult opossum tissues

2025-01-12 | GSE206392 | GEO

snRNA-seq of opossum (Monodelphis domestica) adult testis

Project description:Single-nucleus RNA sequencing (snRNA-seq) was used to profile the transcriptome of 9,926 nuclei in opossum adult testis. This dataset includes three samples from three different individuals. This dataset is part of a larger evolutionary study of adult testis at the single-nucleus level (97,521 single-nuclei in total) across mammals including 10 representatives of the three main mammalian lineages: human, chimpanzee, bonobo, gorilla, gibbon, rhesus macaque, marmoset, mouse (placental mammals); grey short-tailed opossum (marsupials); and platypus (egg-laying monotremes). Corresponding data were generated for a bird (red junglefowl, the progenitor of domestic chicken), to be used as an evolutionary outgroup.

2021-11-08 | E-MTAB-11072 | biostudies-arrayexpress

snRNA-seq of opossum (Monodelphis domestica) adult testis

Project description:snRNA-seq of opossum (Monodelphis domestica) adult testis

| PRJEB48237 | ENA

Gene regulatory divergence in spermatogenesis between mouse and opossum

Project description:Mammalian spermatogenesis is a highly stereotyped and conserved developmental process that is essential for fitness. At the same time, gene expression in spermatogenic cells is rapidly evolving. This combination of features has been suggested to drive rapid fixation of new gene expression patterns. Using a high-resolution dataset comprising bulk and single-cell data from juvenile and adult testes of the opossum Monodelphis domestica, a model marsupial, we define the developmental timing of the spermatogenic first wave in opossum and delineate conserved and divergent gene expression programs across the placental-marsupial split by comparison to equivalent data from mouse, a model placental mammal. Epigenomic data confirmed divergent regulation at the level of transcription, and comparison to data from four additional amniote species identified hundreds of genes with evidence of rapid fixation of expression. This gene set encompasses known and previously undescribed regulators of spermatogenic development.

2024-08-16 | GSE216343 | GEO

T. oceanicus adult tissues

Project description:T. oceanicus adult tissues

| PRJEB27211 | ENA

Transcriptomes of adult mesentery and lung tissues from wildtype and Foxc2lecKO mice

Project description:Bulk RNA sequencing of mesentery and lung tissues from adult willdtype and Foxc2lecKO mice

2021-07-16 | GSE156319 | GEO

Single-cell transcriptomics of infant and adult T cells across human tissues

Project description:We performed bulk and single-cell RNA-seq on resting and stimulated T cells from tissues and blood of infant and adult organ donors.

2025-03-11 | GSE195844 | GEO

RNA-Seq from zebrafish adult tissues

Project description:RNA-Seq from zebrafish adult tissues

| PRJNA721381 | ENA