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Uncultured methanogenic archaeon

ABSTRACT: uncultured methanogenic archaeon overview

PROVIDER: PRJNA851135 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	DRR068623.fastq.gz	Fastqsanger.gz
	DRR068624.fastq.gz	Fastqsanger.gz
	DRR068625.fastq.gz	Fastqsanger.gz
	DRR068626.fastq.gz	Fastqsanger.gz
	DRR068627.fastq.gz	Fastqsanger.gz

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Project description:Biogenic methane formation, methanogenesis, a key process in the global carbon cycle is the only energy metabolism known to sustain growth of the microorganisms employing it, the methanogenic archaea. All known methanogenic pathways converge at the methane-liberating step where also the terminal electron acceptor of methanogenic respiration, the heterodisulfide of coenzyme M and coenzyme B is formed. Carbon monoxide (CO) utilization of Methanosarcina acetivorans is unique in that the organism can shift from methanogenesis towards acetogenesis. Here, we show that M. acetivorans can dispense of methanogenesis for energy conservation completely. By disrupting the methanogenic pathway through targeted mutagenesis, followed by adaptive evolution, a strain capable of sustained growth by CO-dependent acetogenesis was created. Still, a minute flux through the methane-liberating reaction remained essential, which was attributed to the involvement of the heterodisulfide in at least one essential anabolic reaction. Genomic and proteomic analysis showed that substantial metabolic rewiring had occurred in the strain. Most notably, heterodisulfide reductase, the terminal respiratory oxidoreductase was eliminated to funnel the heterodisulfide towards anabolism. These results suggest that the metabolic flexibility of “methanogenic” archaea is much greater than anticipated and open avenues for probing the mechanism of energetic coupling and the crosstalk between catabolism and anabolism.

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