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Gene expression profiling of Human C2 and C1 iNKT cell subset

ABSTRACT: Gene expression profiling of Human C2 and C1 iNKT cell subset

PROVIDER: PRJNA851361 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19762117_1.fastq.gz	Fastqsanger.gz
	SRR19762117_2.fastq.gz	Fastqsanger.gz
	SRR19762118_1.fastq.gz	Fastqsanger.gz
	SRR19762118_2.fastq.gz	Fastqsanger.gz
	SRR19762119_1.fastq.gz	Fastqsanger.gz

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Gene expression profiling of C2, C1 and C0 iNKT cell subset

Project description:We identified a novel subset of iNKT cells, C2 iNKT cells, that circulate in the periphery. Correspondingly, we characterized the tissue-resident iNKT cell subset, C1 iNKT cells. We also found the precursor of these two subsets of iNKT cells, C0 iNKT cells in thymus. The development and terminal maturation of C2 iNKT cells completely depended on the thymic epithelial IL-15 niche, whereas C1 iNKT cells were regulated also by local IL-15 niches in peripheral tissues. C2 iNKT cells expressed high levels of genes related to cytotoxicity and exhibited more NK cell-like features. Here we characterized the C2 iNKT cells, C1 iNKT cells, and C0 iNKT cells using RNA-seq. We also performed RNA-seq for CD4+ T cells, CD8+ T cells and NK cells as a comparison. To investigate the effect of CD4, we performed the RNA-seq for the CD4+ and CD4- C2 iNKT cells.

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Project description:We identified two novel subsets of iNKT cells in mouse, C2 iNKT cells (the circulating subset) and C1 iNKT cells (the tissue-resident subset). Here, we characterized the cells of these two subsets in human peripheral blood.

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Project description:Gene expression profiling of C2, C1 and C0 iNKT cell subset

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