Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mouse gut metagenome

ABSTRACT: Antibiotics treated mouse fecal bacterial metagenome

PROVIDER: PRJNA851488 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR19776765_1.fastq.gz	Fastqsanger.gz
	SRR19776765_2.fastq.gz	Fastqsanger.gz
	SRR19776766_1.fastq.gz	Fastqsanger.gz
	SRR19776766_2.fastq.gz	Fastqsanger.gz
	SRR19776767_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

mouse gut metagenome

Project description:Antibiotics treated mouse fecal bacteria Metagenome

| PRJNA507690 | ENA

mouse gut metagenome

Project description:Mouse fecal metagenome

| PRJNA289572 | ENA

mouse gut metagenome

Project description:Mouse Fecal Samples Metagenome

| PRJNA277975 | ENA

mouse gut metagenome

Project description:Mice fecal lipidomics in antibiotics treatment

| PRJDB9922 | ENA

mouse gut metagenome

Project description:Mouse gut metagenome of HFD mice after receiving Ganoderma lucidum treated fecal transplant

| PRJNA284556 | ENA

mouse gut metagenome

Project description:Low complexity mouse metagenome from fecal sample

| PRJNA219419 | ENA

mouse gut metagenome

Project description:AD model transgenic mouse fecal bacteria Metagenome

| PRJNA449431 | ENA

Depletion of murine intestinal microbiota: effects on gut mucosa and epithelial gene expression

Project description:Inappropriate cross talk between mammals and their gut microbiota may trigger intestinal inflammation and drive extra-intestinal immune-mediated diseases. Studies with germ-free or gnotobiotic animals represent the gold standard for research on bacterial-host interaction but they are not readily accessible to the wide scientific community. We aimed at refining a protocol that in a robust manner would deplete murine intestinal microbiota and prove to have significant biologic validity. Previously published protocols for depleting mice of their intestinal microbiota by administering broad-spectrum antibiotics in drinking water were difficult to reproduce. We show that twice daily delivery of antibiotics by gavage depleted mice of their cultivable fecal microbiota and reduced the fecal bacterial DNA load by approximately 400 fold while ensuring the animals’ health. Mice subjected to the protocol for 17 days displayed enlarged ceca, reduced Peyer’s patches and small spleens. Antibiotic treatment significantly reduced the expression of antimicrobial factors and altered the expression of 517 genes in total in the colonic epithelium. Genes involved in cell cycle were significantly altered concomitant with reduced epithelial proliferative activity in situ assessed by Ki-67 expression, suggesting that commensal microbiota drives cellular proliferation in colonic epithelium. We present a robust protocol for depleting mice of their cultivatable intestinal microbiota with antibiotics by gavage and show that the biological effect of this depletion is phenotypic characteristics and epithelial gene expression profile similar to those of germ-free mice. Comparison of genome-wide gene expression of colon intestinal epithelial cells from mice subjected to microbiota depletion protocol against to control mice.

2011-08-14 | E-GEOD-22648 | biostudies-arrayexpress

mouse gut metagenome

Project description:Diversity Outbred Mouse Fecal Metagenome Raw Sequence Reads

| PRJNA486846 | ENA

mouse gut metagenome

Project description:mouse gut metagenome after fecal or cecal transplant

| PRJNA350534 | ENA