Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A glycan-based approach to cell characterization and isolation (scRNA-Seq)

ABSTRACT: A glycan-based approach to cell characterization and isolation (scRNA-Seq)

PROVIDER: PRJNA851753 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

A glycan-based approach to cell characterization and isolation

Project description:A glycan-based approach to cell characterization and isolation

| PRJNA851746 | ENA

A glycan-based approach to cell characterization and isolation (bulk RNA-Seq)

Project description:A glycan-based approach to cell characterization and isolation (bulk RNA-Seq)

| PRJNA851754 | ENA

A glycan-based approach to cell characterization and isolation (scRNA-Seq)

Project description:We leverage the extraordinary molecular diversity of modified heparan sulfate (HS) glycans 8 to establish cellular glycotypes, defined by binding patterns of a panel of flow-cytometry compatible single-chain variable fragment antibodies (scFvs) specific for differentially modified HS. We find distinct glycotypes between closely related hematopoietic progenitors and lineages. The glycotypes of murine and human hematopoietic stem and progenitor cells (HSPCs) reveal dynamic yet similar HS modification patterns in vivo and in vitro, including along megakaryocyte and erythrocyte differentiation. Prospective HS scFv-based sorting identifies new cellular subtypes from both immunophenotypic megakaryocyte-erythrocyte progenitors and heterogeneous pools of HSPCs, thus offering additional discriminative power beyond conventional CD markers. Mechanistically, single-cell RNAseq revealed that a heptad of HS-related genes participate in megakaryocyte-erythrocyte fate determination and are reflective of the HS epitope recognized by specific HS scFvs. In summary, HS glycotyping establishes a role for HS modification patterns in hematopoietic lineage differentiation in mouse and human, and provides an orthogonal approach to define and isolate viable cell types across different cell lineages and species at unprecedented resolution.

2022-08-24 | GSE206671 | GEO

A glycan-based approach to cell characterization and isolation

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-08-24 | GSE206672 | GEO

A glycan-based approach to cell characterization and isolation (bulk RNA-Seq)

Project description:We leverage the extraordinary molecular diversity of modified heparan sulfate (HS) glycans to establish cellular glycotypes, defined by binding patterns of a panel of flow-cytometry compatible single-chain variable fragment antibodies (scFvs) specific for differentially modified HS. We find distinct glycotypes between closely related hematopoietic progenitors and lineages. The glycotypes of murine and human hematopoietic stem and progenitor cells (HSPCs) reveal dynamic yet similar HS modification patterns in vivo and in vitro, including along megakaryocyte and erythrocyte differentiation. Prospective HS scFv-based sorting identifies new cellular subtypes from both immunophenotypic megakaryocyte-erythrocyte progenitors and heterogeneous pools of HSPCs, thus offering additional discriminative power beyond conventional CD markers. Mechanistically, single-cell RNAseq revealed that a heptad of HS-related genes participate in megakaryocyte-erythrocyte fate determination and are reflective of the HS epitope recognized by specific HS scFvs. In summary, HS glycotyping establishes a role for HS modification patterns in hematopoietic lineage differentiation in mouse and human, and provides an orthogonal approach to define and isolate viable cell types across different cell lineages and species at unprecedented resolution.

2022-08-24 | GSE206670 | GEO

Transcriptomic profilling of dental pericytes, an RNAseq approach [scRNA-Seq]

Project description:Transcriptomic profilling of dental pericytes, an RNAseq approach [scRNA-Seq]

| PRJNA634140 | ENA

Development of a glycan microarray based method for ABO blood typing

Project description:Purpose: This study uses a high-throughput glycan microarray to develop a novel method to assign ABO blood type. The method will then be applied to samples from patients treated with PROSTVAC to determine if blood type correlates with survival Results: Many blood group A and B antigens correlate with blood type. Blood typing is best achieved using a combination of 10 signals Conclusion: ABO blood type can be determined with greater than 97% accuracy using only 4 microliters of serum.

2016-04-28 | GSE68403 | GEO

Identification and isolation of hematopoietic stem and progenitor cell populations (scRNA-seq)

Project description:Identification and isolation of hematopoietic stem and progenitor cell populations (scRNA-seq)

| PRJNA706739 | ENA

Streptococcus pneumoniae membrane proteomics of WT and scRNA mutants

Project description:We studied the membrane protein compositions of Streptococcus pneumoniae WT and scRNA mutant strains, with or without the CSP1 induction into competence state.

2024-08-19 | PXD054463 | Pride

Glycan effect on Saccharolytic Human Distal Gut Symbiont, B. thetaiotaomicron

Project description:This SuperSeries is composed of the following subset Series: GSE11944: Mucosal Glycan Foraging Enhances the Fitness and Transmission of a Saccharolytic Human Distal Gut Symbiont GSE11953: Mucosal Glycan Foraging Enhances the Fitness and Transmission of a Saccharolytic Human Distal Gut Symbiont: ECF mutant GSE11962: Growth of B. thetaiotaomicron on purified host mucosal glycans and glycan fragments Refer to individual Series

2008-11-20 | E-GEOD-11980 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data