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RNA Polymerase II transcription independent of TBP (CUT&Tag)

ABSTRACT: RNA Polymerase II transcription independent of TBP (CUT&Tag)

PROVIDER: PRJNA851783 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23950900_1.fastq.gz	Fastqsanger.gz
	SRR23950900_2.fastq.gz	Fastqsanger.gz
	SRR23950901_1.fastq.gz	Fastqsanger.gz
	SRR23950901_2.fastq.gz	Fastqsanger.gz
	SRR23950902_1.fastq.gz	Fastqsanger.gz

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RNA Polymerase II transcription independent of TBP (CUT&Tag)

Project description:Using CUT&Tag, a chromatin profiling technique, we show that TBP depletion via IAA surprisingly does not affect RNA Pol II transcription but affects RNA Pol III transcription. Additionally, induction of genes via heat shock and retinoic acid treatment does not require TBP. We also show that a metazoan specific paralog TRF2 does not compensate for TBP for RNA Pol II transcription and that the TFIID subunit of the Pre-initiation Complex can still form with specific subunits still binding onto DNA when TBP is depleted.

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