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Context-dependent function of long noncoding RNA PURPL in transcriptome regulation during p53 activation (Iso-Seq)

ABSTRACT: Context-dependent function of long noncoding RNA PURPL in transcriptome regulation during p53 activation (Iso-Seq)

PROVIDER: PRJNA851801 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19785215_subreads.fastq.gz	Fastqsanger.gz
	SRR19785216_subreads.fastq.gz	Fastqsanger.gz

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Context-dependent function of long noncoding RNA PURPL in transcriptome regulation during p53 activation (Iso-Seq)

Project description:The goal of our study is to investigate the role of the p53-induced lncRNA PURPL in the context of liver cancer. To achieve this, we identified transcript variants of PURPL expressed in liver cancer cells and assayed for the transcriptome changes caused by PURPL knockdown after p53 induction.

2022-10-18 | GSE206701 | GEO

Context-dependent function of long noncoding RNA PURPL in transcriptome regulation during p53 activation (RNA-Seq II)

2022-10-18 | GSE206699 | GEO

Context-dependent function of long noncoding RNA PURPL in transcriptome regulation during p53 activation (RNA-Seq I)

2022-10-18 | GSE206698 | GEO

Context-dependent function of long noncoding RNA PURPL in transcriptome regulation during p53 activation

Project description:Context-dependent function of long noncoding RNA PURPL in transcriptome regulation during p53 activation

| PRJNA851788 | ENA

Context-dependent function of long noncoding RNA PURPL in transcriptome regulation during p53 activation (RNA-Seq I)

Project description:Context-dependent function of long noncoding RNA PURPL in transcriptome regulation during p53 activation (RNA-Seq I)

| PRJNA851785 | ENA

Context-dependent function of long noncoding RNA PURPL in transcriptome regulation during p53 activation (RNA-Seq II)

Project description:Context-dependent function of long noncoding RNA PURPL in transcriptome regulation during p53 activation (RNA-Seq II)

| PRJNA851791 | ENA

Identification of PURPL regulated genes in HCT116 cells

Project description:Analysis of changes in gene expression profile upon depletion of PURPL in HCT116 cells. The hypothesis in this study is that PURPL regulates gene expression in the p53 pathway.

2017-08-03 | GSE79053 | GEO

PURPL

Project description:Homo sapiens PURPL, p53 upregulated regulator of p53 levels [Source:HGNC Symbol;Acc:HGNC:48995], is differentially expressed in 63 experiment(s);

| ENSG00000250337 | ExpressionAtlas

PURPL

Project description:Homo sapiens PURPL, p53 upregulated regulator of p53 levels [Source:HGNC Symbol;Acc:HGNC:48995], is expressed in 27 baseline experiment(s);

| ENSG00000250337 | ExpressionAtlas

Context-Dependent Function of Long Noncoding RNA <i>PURPL</i> in Transcriptome Regulation during p53 Activation.

Project description:PURPL is a p53-induced lncRNA that suppresses basal p53 levels. Here, we investigated PURPL upon p53 activation in liver cancer cells, where it is expressed at significantly higher levels than other cell types. Using isoform sequencing, we discovered novel PURPL transcripts that have a retained intron and/or previously unannotated exons. To determine PURPL function upon p53 activation, we performed transcriptome sequencing (RNA-Seq) after depleting PURPL using CRISPR interference (CRISPRi), followed by Nutlin treatment to induce p53. Strikingly, although loss of PURPL in untreated cells altered the expression of only 7 genes, loss of PURPL resulted in altered expression of ~800 genes upon p53 activation, revealing a context-dependent function of PURPL. Pathway analysis suggested that PURPL is important for fine-tuning the expression of specific genes required for mitosis. Consistent with these results, we observed a significant decrease in the percentage of mitotic cells upon PURPL depletion. Collectively, these data identify novel transcripts from the PURPL locus and suggest that PURPL delicately moderates the expression of mitotic genes in the context of p53 activation to control cell cycle arrest.

| S-EPMC9753727 | biostudies-literature

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