Project description:We developed a serum and animal component-free medium capable of supporting robust growth of the genome-reduced bacterium Mycoplasma pneumoniae. Here, we analyzed the proteome of the wild-type strain grown in serum-free vB13 medium compared to rich medium (Hayflick medium).
Project description:Overall design Hi-C experiments were performed on untreated wild type cells at stationary and drug-treated cells (Novobiocin) of Mycoplasma Pneumoniae MPN129. We studied the chromosome organization of the genome-reduced bacterium, Mycoplasma pneumoniae, which has minimal genetic components and lacks several structural DNA-binding proteins. Platforms : Illumina HiSeq 2000 (Mycoplasma Pneumoniae MPN129)
Project description:The NifA-RpoN complex is a master regulator of the nitrogen fixation genes in alpha-proteobacteria. Based on the complete Rhizobium etli genome sequence, we constructed the R. etli CFN42 oligonucleotide (70 mer) microarray, and utilized this tool to survey changes in gene expression in R. etli CFN42 wild type compared with NifA CFNX247 mutant strain in symbiosis with Phaseolus vulgaris. As expected, the genes associated with a NifA and RpoN binding sites were downregulated in the NifA mutant strain.