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Brachypodium hybridum cultivar:Bhyb26

ABSTRACT: Brachypodium hybridum Bhyb26 Transcriptome - Bhyb26_root

PROVIDER: PRJNA852517 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR20045868_subreads.fastq.gz	Fastqsanger.gz
	SRR20045871_subreads.fastq.gz	Fastqsanger.gz
	SRR20045874_1.fastq.gz	Fastqsanger.gz
	SRR20045874_2.fastq.gz	Fastqsanger.gz

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Centromere evolution in Brachypodium genomes with different polyploid levels

Project description:Centromeric repetitive DNA sequences are highly variable during evolution, which are the hub for genome stability in almost all the eukaryotic organisms. However, how centromeric repeat sequences diverge rapidly among closely related species and populations, and how polyploidy contributed to the diversity of centromere among co-evolved subgenomes are largely unknown. Here, we applied the Brachypodium system to investigate the track of centromere evolution within this taxa, and their adaptation to alloploidization process. Subgenome divergent centromeric satellite repeat were discovered in tetraploid B. hybridum, and this divergent was originated form their two diploid progenitors. Furthermore, differential sequences influence the association sites with CENH3 nucleosomes on the monomer satellite repeats, and positioning of CENH3 nucleosomes on the satellite DNA are stable in each subgenome after alloploidization. Only minor intrasubgenomic variations were observed on these satellite repeats from diploid to tetraploid in B. hybridum, and no evident intersubgenomic transfer of centromeric satellite repeats after alloploidization. Pan-genome analysis reveals that the general principle of centromere dynamic within the populations in Brachypodium genomes with different polyploidy level. Our results provide an unprecedented information regarding the genomic and functional diversity of centromeric repeat DNA during evolution.

2022-12-01 | GSE157143 | GEO

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