Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA sequencing on EphrinB2 overexpression cardiac fibroblasts and vector cardiac fibroblasts within or without induction of osteogenic differentiation in the 7th day.

ABSTRACT: RNA sequencing on EphrinB2 overexpression cardiac fibroblasts and vector cardiac fibroblasts within or without induction of osteogenic differentiation in the 7th day.

PROVIDER: PRJNA852948 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR19852568_1.fastq.gz	Fastqsanger.gz
	SRR19852568_2.fastq.gz	Fastqsanger.gz
	SRR19852569_1.fastq.gz	Fastqsanger.gz
	SRR19852569_2.fastq.gz	Fastqsanger.gz
	SRR19852570_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

RNA sequencing on EphrinB2 overexpression cardiac fibroblasts and vector cardiac fibroblasts within or without induction of osteogenic differentiation in the 7th day.

Project description:Purpose: To investigate the function of EphrinB2 in the regulation of osteogenic differentiation of cardiac fibroblasts, we established EphrinB2-overexpressing cardiac fibroblasts and corresponding control group by lentivirus.Methods:Adult cardiac fibroblasts were isolated from 6 to 8-week-aged C57BL/6 mice, passage 1 cardiac fibroblasts plated at a density of 2.5 x 104 cells/cm2 were infected with the EphrinB2-overexpressing or vector lentivirus at an MOI of 20 for 12h, after 48 hours’ infection, osteogenesis was induced using differentiation medium (α-MEM supplied with 10% FBS, 10 mM β-glycerophosphate, 100nM dexamethasone and 50 µg/mL L-ascorbic acid). Cardiac fibroblasts harvested at Day7 were used for RNA sequencing.

2022-06-29 | GSE206947 | GEO

RNA sequencing on cardiac fibroblasts harvested at different time points following induction of osteogenic differentiation

Project description:Purpose: To analyze cardiac fibroblasts gene expression at different time following induction of osteogenic differentiation Methods: Freshly isolated cardiac fibroblasts (Passage 0 or passage 1) were plated at a density of 2.5 x 104 cells/cm2 in growth medium. After overnight incubation, osteogenesis was induced using differentiation medium (α-MEM supplemented with 10% FBS, 10 nM dexamethasone (Sigma, D4902), 20 mM β-glycerol phosphate (Sigma, G9422), and 50 μM L-ascorbic acid (Sigma, A4403). Cardiac fibroblasts harvested at Day0 (before differentiation medium treatment), Day7, Day14 and Day21 were used for RNA sequencing. Results: Cardiac fibroblasts harvested at different time points following induction of differentiation revealed clusters of genes whose expression was significantly altered in a temporal specific manner. Genes regulating cell cycle that were highly expressed in undifferentiated cardiac fibroblasts were down-regulated at the onset of differentiation and remained at low expression levels throughout the duration of osteogenic differentiation, consistent with the principle that induction of differentiation is associated with reduced rates of proliferation. In contrast, genes that were minimally expressed in cardiac fibroblasts were induced in a specific temporal manner during the course of osteogenic differentiation and included sets of genes known to regulate inflammation, extracellular matrix proteins and cell metabolism. Conclusions: Cardiac fibroblasts subjected to osteogenic differentiation progressively adopted an osteogenic signature.

2016-11-07 | GSE87836 | GEO

Global gene expression in adult cardiac fibroblasts transduced with cardiac transcription factor

Project description:Four transcription factors, GATA4, Hand2, MEF2C, Tbx5 (GHMT) activated cardiac gene expression in cardiac fibroblasts, suggesting that these factors are able to reprogram fibroblasts toward a cardaic cell fate. Total RNA isolated from adult cardiac fibroblasts transduced with empty retroviral vector or GHMT-retroviruses for 2, and 4 weeks.

2012-04-05 | E-GEOD-37057 | biostudies-arrayexpress

Chemically-induced osteogenic cells (ciOG) from fibroblasts

Project description:Here we report the reprogramming of human fibroblasts to produce chemically-induced osteogenic cells (ciOG), and explore the potential uses of ciOG in bone repair and disease treatment. A chemical cocktail of RepSox, forskolin, and phenamil was used for osteogenic induction of fibroblasts by activation of RUNX2 expression. Following a maturation, the cells differentiated toward an osteoblast phenotype that produced mineralized tissue. Bulk RNA sequencing revealed that up- and down-regulated genes in ciOG relative to aHDF resembled those in hOB during maturation.

2022-09-15 | GSE206136 | GEO

Chemically-induced osteogenic cells (ciOG) from fibroblasts

Project description:Chemically-induced osteogenic cells (ciOG) from fibroblasts

| PRJNA849333 | ENA

Chitin-binding peptides from rat cardiac fibroblasts

Project description:Chitin-binding peptides from rat cardiac fibroblasts

2023-12-26 | PXD039099 | JPOST Repository

In-depth transcriptomic analyses investigating molecular mechanisms underlying the osteogenic differentiation of human renal interstitial fibroblasts.

Project description:Randall’s plaques (RP) are well established as precursor lesions of idiopathic calcium oxalate (CaOx) stones, and the process of biomineralization driven by osteogenic-like cells has been highlighted in RP formation, but the mechanism is poorly understood. Given the potential role of osteogenic-like renal interstitial fibroblasts in biomineralization, the isolated primary human renal interstitial fibroblasts (hRIFs) were either induced with a widely used osteogenic medium or cultured in normal medium for 7 days, and a transcriptomic analysis of LncRNA and mRNA was performed to study molecular mechanisms underlying the osteogenic differentiation of human renal interstitial fibroblasts.

2022-05-19 | GSE203110 | GEO

Mouse Cardiac Fibroblasts post MI

Project description:Analysis of one-day cultured cardiac fibroblasts after acute myocardial infarction

2020-05-20 | MSV000085454 | MassIVE

Effect of HOXB13 overexpression on osteogenic differentiation in mouse embryonic fibroblasts (MEFs)

Project description:Studies of the effect of HOXB13 overexpression on mesenchymal stem cell (MSC) marker expression and osteogenic differentiation in mouse embryonic fibroblasts (MEFs)

2020-02-25 | GSE145813 | GEO

Robust Cardiac Reprogramming Systems with Selectivity to Cardiac Fibroblasts

Project description:Robust Cardiac Reprogramming Systems with Selectivity to Cardiac Fibroblasts

| PRJNA878785 | ENA