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ZBTB11 and SET regulates genes in NCI-H1299

ABSTRACT: ZBTB11 and SET regulates genes in NCI-H1299

PROVIDER: PRJNA853050 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19854836_1.fastq.gz	Fastqsanger.gz
	SRR19854836_2.fastq.gz	Fastqsanger.gz
	SRR19854837_1.fastq.gz	Fastqsanger.gz
	SRR19854837_2.fastq.gz	Fastqsanger.gz
	SRR19854838_1.fastq.gz	Fastqsanger.gz

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ZBTB11 and SET regulates genes in NCI-H1299

Project description:To determined ZBTB11 and SET regulates genes in NCI-H1299, we esteblished NCI-H1299 cell lines in which ZBTB11 and SET has been knocked down by si-RNA. We then conducted differential expressed genes analysis using data generated form RNA-seq of H1299 cell lines at the condition of two genes knocked down.

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Project description:Gene expression for NCI-H1299 cell line transfected with human DENND2D and vector (pcDNA3.1/V5-His TOPO TA vector) respectively. The microarray experiment was designed to perform four replicates for each H1299-DENND2D and H1299-vector sample. cRNA used in replication 1 and 2 was from the same label reaction to perform the hybridization replicates.

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Project description:Gene expression in NCI-H1299 cells infected with shCtrl and shRPL35A was detected with a PrimeView human gene expression array. Gene expression profiling of shCtrl and shRPL35A NCI-H1299 cells was acquired and analyzed. Differentially expressed genes were identified based on fold change of mean of expression (fold change ≥ 1.3) and FDR (< 0.05) from P value calculated based on linear model of empirical Bayesian distribution. Finally, 2055 upregulated genes and 1559 downregulated genes were characterized.

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Homo sapiens strain:NCI-H1299

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