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To find target genes of Zeb1 [CUT&Tag-Seq]

ABSTRACT: To find target genes of Zeb1 [CUT&Tag-Seq]

PROVIDER: PRJNA853063 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19854958_1.fastq.gz	Fastqsanger.gz
	SRR19854958_2.fastq.gz	Fastqsanger.gz
	SRR19854959_1.fastq.gz	Fastqsanger.gz
	SRR19854959_2.fastq.gz	Fastqsanger.gz
	SRR19854960_1.fastq.gz	Fastqsanger.gz

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To find target genes of Zeb1 [CUT&Tag-Seq]

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Bulk Cut&run and Cut&tag

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scNanoSeq-CUT&Tag: a long-read single-cell CUT&Tag sequencing method for efficient chromatin modification profiling within individual cells

Project description:We developed scNanoSeq-CUT&Tag, a streamlined method by adapting a modified CUT&Tag protocol to Oxford Nanopore sequencing platform for efficient chromatin modification profiling at single-cell resolution. We firstly tested the performance of scNanoSeq-CUT&Tag on six human cell lines: K562, 293T, GM12878, HG002, H9, HFF1 and adult mouse blood cells, it showed that scNanoSeq-CUT&Tag can accurately distinguish different cell types in vitro and in vivo. Moreover, scNanoSeq-CUT&Tag enables to effectively map the allele-specific epigenomic modifications in the human genome andallows to analyze co-occupancy of histone modifications. Taking advantage of long-read sequencing,scNanoSeq-CUT&Tag can sensitively detect epigenomic state of repetitive elements. In addition, by applying scNanoSeq-CUT&Tag to testicular cells of adult mouse B6D2F1, we demonstrated that scNanoSeq-CUT&Tag maps dynamic epigenetic state changes during mouse spermatogenesis. Finally, we exploited the epigenetic changes of human leukemia cell line K562 during DNA demethylation, it showed that NanoSeq-CUT&Tag can capture H3K27ac signals changes along DNA demethylation. Overall, we prove that scNanoSeq-CUT&Tag is a valuable tool for efficiently probing chromatin state changes within individual cells.

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