Project description:RATIONALE: Studying the genes expressed in samples of tissue from patients with cancer may help doctors identify biomarkers related to cancer. PURPOSE: This laboratory study is using gene expression profiling to evaluate normal tissue and tumor tissue from patients with colon cancer that has spread to the liver, lungs, or peritoneum.

| 2071958 | ecrin-mdr-crc

Translational profiling of hypothalamic and midbrain neurons that project to the nucleus accumbens.

Project description:Neuroanatomical methods enable high-resolution mapping of neural circuitry, but do not allow systematic molecular profiling of neurons based on their connectivity. Here, we report the development of a novel approach for molecularly profiling projective neurons. We show that ribosomes can be labeled with a camelid nanobody raised against GFP and that this system can be engineered to selectively capture translating mRNAs from cells expressing GFP. We generated a transgenic mouse encoding a nanobody-ribosomal protein fusion (Syn-NBL10) and used a retrograde virus (CAV) encoding GFP to immunoprecipitate ribosomes from projection neurons. This enabled us to profile neurons projecting to the nucleus accumbens. The current method provides a new means for profiling neurons based on their projections. Translating mRNAs immunoprecipitated from neurons projecting to the nucleus accumbens. Each Input and IP sample corrspond to a pooled group of 6 mice.

2014-05-22 | E-GEOD-55800 | biostudies-arrayexpress

Gene Expression Profiling

Project description:Gene Expression Profiling from 21 cases: 14 CCND1-negative Mantle Cell Lymphoma and 7 CCND1-positive Mantle Cell Lymphoma

| EGAD00010001582 | EGA

Gene expression profiling of Calca neurons in the parabrachial nucleus (PBN)

Project description:Calca-expressing neurons in the parabrachial nucleus (PBN) play a major role in regulating appetite and transmitting real or potential threat signals. Here, we reveal transcripts specific or highly enriched in this neuronal population by using mouse genetics and a conditional viral ribosome-tagging approach to phenotype these neurons.

2022-07-02 | GSE207153 | GEO

Gene expression profiling of shRNA transduced rat hippocampal neurons

Project description:Gene expression profiling of shRNA transduced rat hippocampal neurons

| PRJNA642132 | ENA

Gene Expression Profiling for study EGAS00001004165

Project description:Gene Expression Profiling from 44 Mantle Cell Lymphoma cases

| EGAD00010001842 | EGA

Translational profiling of hypothalamic and midbrain neurons that project to the nucleus accumbens.

2014-05-22 | GSE55800 | GEO