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Multi-hallmark long noncoding RNA maps reveal non-small cell lung cancer vulnerabilities [CRISPRseries]

ABSTRACT: Multi-hallmark long noncoding RNA maps reveal non-small cell lung cancer vulnerabilities [CRISPRseries]

PROVIDER: PRJNA854197 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19903619_1.fastq.gz	Fastqsanger.gz
	SRR19903619_2.fastq.gz	Fastqsanger.gz
	SRR19903620_1.fastq.gz	Fastqsanger.gz
	SRR19903620_2.fastq.gz	Fastqsanger.gz
	SRR19903621_1.fastq.gz	Fastqsanger.gz

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Multi-hallmark long noncoding RNA maps reveal non-small cell lung cancer vulnerabilities.

Project description:Multi-hallmark long noncoding RNA maps reveal non-small cell lung cancer vulnerabilities.

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Multi-hallmark long noncoding RNA maps reveal non-small cell lung cancer vulnerabilities [CRISPRseries]

Project description:Long noncoding RNAs (lncRNAs) are widely dysregulated in cancer, yet their functional roles in cellular disease hallmarks remain unclear. Here we employ pooled CRISPR deletion to perturb 831 lncRNAs detected in KRAS-mutant non-small cell lung cancer (NSCLC), and measure their contribution to proliferation, chemoresistance and migration across two cell backgrounds. Integrative analysis of this data outperforms conventional “dropout” screens in identifying cancer genes, while prioritising disease-relevant lncRNAs with pleiotropic and background-independent roles. Altogether 80 high-confidence oncogenic lncRNAs are active in NSCLC, the majority identified here for the first time, and which tend to be amplified and overexpressed in tumours. A follow-up antisense oligonucleotide (ASO) screen shortlisted two candidates, Cancer Hallmarks in Lung LncRNA 1 (CHiLL 1) and GCAWKR, whose knockdown consistently suppressed cancer hallmarks in a variety of two- and three-dimension tumour models. Molecular phenotyping reveals that CHiLL 1 and GCAWKR control cellular-level phenotypes via distinct transcriptional networks converging on common oncogenic pathways. In summary, this work reveals a multi-dimensional functional lncRNA landscape underlying NSCLC that contains potential therapeutic vulnerabilities.

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Project description:Long noncoding RNAs (lncRNAs) are widely dysregulated in cancer, yet their functional roles in cancer hallmarks remain unclear. We employ pooled CRISPR deletion to perturb 831 lncRNAs detected in KRAS-mutant non-small cell lung cancer (NSCLC) and measure their contribution to proliferation, chemoresistance, and migration across two cell backgrounds. Integrative analysis of these data outperforms conventional "dropout" screens in identifying cancer genes while prioritizing disease-relevant lncRNAs with pleiotropic and background-independent roles. Altogether, 80 high-confidence oncogenic lncRNAs are active in NSCLC, which tend to be amplified and overexpressed in tumors. A follow-up antisense oligonucleotide (ASO) screen shortlisted two candidates, Cancer Hallmarks in Lung LncRNA 1 (CHiLL1) and GCAWKR, whose knockdown consistently suppressed cancer hallmarks in two- and three-dimension tumor models. Molecular phenotyping reveals that CHiLL1 and GCAWKR control cellular-level phenotypes via distinct transcriptional networks. This work reveals a multi-dimensional functional lncRNA landscape underlying NSCLC that contains potential therapeutic vulnerabilities.

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