Acinetobacter baumannii
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA854605 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Acinetobacter baumannii is a pathogen, highly adaptable to both hospital and host-associated environments. Understanding the genetic mechanisms underlying its adaptability is critical for controlling its persistence. Our study identified the crp-osmC gene cluster, flanked by ISAba1 elements, as a significant genetic structure undergoing amplification in A. baumannii. By experimentally mimicking the amplification of this gene cluster, we found that it substantially enhanced bacterial growth and competitiveness, while also increasing biofilm formation and serum resistance. Furthermore, amplification of the crp-osmC cluster enhanced colonization in murine infection models. Notably, this amplification was largely limited to the epidemic ST2 lineage clinical isolates. Intriguingly, gene amplification was also accompanied by heightened susceptibility to several antibiotics. Overall, ISAba1-mediated amplification of the crp-osmC cluster enhances host-associated fitness in A. baumannii, while the accompanying increase in antibiotic sensitivity reflects a trade-off between survival and resistance.
2026-05-20 | GSE331122 | GEO
Project description:Cefiderocol (CFDC) is a novel chlorocatechol-substituted siderophore approved to treat complicated urinary tract infections and for hospital-acquired and ventilator-acquired pneumonia. In previous work, human fluids, were shown to increase the minimum inhibitory concentration (MICs) of Acinetobacter baumannii against CFDC and reduce the expression of genes related to iron uptake systems, which could explain the need for higher concentrations of CFDC to exert inhibitory action. Herein, we analyzed the impact of human urine (HU), which contains low albumin concentrations, on the expression of iron-uptake related genes and MIC values of two carbapenem-resistant A. baumannii. Levels of resistance to CFDC were not modified by HU in strain AMA40 but were reduced in the case of strain AB5075. Testing other carbapenem-resistant A. baumannii isolates showed that the CFDC MICs were unmodified or reduced in the presence of HU. The expression of piuA, pirA, bauA, and bfnH determined by qRT-PCR was enhanced in both strains when HU was present in the culture medium. All four tested genes are involved in recognizing ferric siderophore complexes or internalization into the cell’s cytosol. In contrast, the effect of HU on genes associated with resistance to β-lactams, antibiotics commonly used to treat urinary tract infections caused by A. baumannii, was variable; the transcriptional analysis of pbp1, pbp3, blaOXA-51-like, blaADC, and blaNDM-1 showed significant variation. In summary, HU, probably due to the albumin and free iron content, does not adversely impact or slightly improves the activity of CFDC when tested against A. baumannii in urine in contrast to other human bodily fluids.
2022-06-01 | GSE201259 | GEO
Project description:Carbapenem resistant Acinetobacter baumannii clinical isolates
| PRJNA1507525 | ENA
Project description:Proteomic analysis of Acinetobacter baumannii grown at two different incubation temperatures
2017-10-25 | MSV000081647 | MassIVE
Project description:A major reservoir for spread of the emerging pathogen Acinetobacter baumannii is hopsital surfaces, where bacteria persist in a desiccated state. To identify gene products influencing desiccation survival, a transposon sequencing (Tn-seq) screen was performed. Using this approach, we identified genes both positively and negatively impacting the desiccation tolerance of A. baumannii.
2022-03-09 | GSE198004 | GEO
Project description:Asymptomatic gut colonization increases the risk of clinical infection and transmission by the multidrug-resistant pathogen Acinetobacter baumannii. Ornithine utilization was shown to be critical for A. baumannii competition with the resident microbiota to persist in gut colonization, but the regulatory mechanisms and cues are unknown. Here, we identify a transcriptional regulator, AstR, that specifically activates the expression of the A. baumannii ornithine utilization operon astNOP. Phylogenetic analysis suggests that AstR was co-opted from the Acinetobacter arginine utilization ast(G)CADBE locus and is specialized to regulate ornithine utilization in A. baumannii. Reporter assays showed that astN promoter expression was activated by ornithine but inhibited by glutamate and other preferred amino acids. astN promoter expression was similarly activated by incubation with fecal samples from conventional mice but not germ-free mice, suggesting AstR-dependent activation of the astN promoter responds to intermicrobial competition for amino acids. Finally, AstR was required for A. baumannii to colonize the gut in a mouse model. Together, these results suggest that pathogenic Acinetobacter species evolved AstR to regulate ornithine catabolism, which is required to compete with the microbiota during gut colonization.
2025-09-19 | GSE308136 | GEO
Project description:RNA sequencing was used to analyze the transcriptome of a thioredoxin-A knockout of a clinical isolate of Acinetobacter baumannii
2019-01-14 | GSE125017 | GEO
Project description:Comparison for gene expression of Acinetobacter baumannii NCCP 16007 by evolved strains
2022-11-16 | GSE217840 | GEO
Project description:We performed RNAseq for gene expression analysis for six strains of Acinetobacter Baumannii isolated from blood samples (defined as strains 1, 2, 3, 4 and 6) of patients hospitalized at the University Hospital \\"San Giovanni di Dio e Ruggi d'Aragona\\" (Salerno, Italy)
2023-09-01 | E-MTAB-13274 | biostudies-arrayexpress