Project description:To identify specific gene networks induced in host roots by C. geophilum, we inoculated seedlings of Scots pine simultaneously with C. geophilum and either Suillus granulatus or Rhizopogon roseolus, two common ECM fungi associated to pines. We then measured the differential expression of Scots pine genes in the respective mycorrhizas using oligoarrays.

2016-08-01 | GSE80549 | GEO

Ips pini JHIII male & female timecourse

Project description:This series examines gene expression in the anterior midgut at several time points (2, 4, 8, & 16 h) after topical application of juvenile hormone III (JHIII) or acetone (control) to adult beetles of both sexes. Keywords = North American pine engraver beetle Keywords = anterior midgut Keywords = juvenile hormone Keywords = pheromone biosynthesis Keywords = Coleoptera Keywords = Scolytidae Keywords: other

2005-06-16 | GSE1483 | GEO

Transcript profiling of Pinus sylvestris trees as a response to Heterobasidion annosum infection under field conditions

Project description:In conifer forests of Northern Europe, a pathogenic fungus Heterobasidion annosum attacks the roots of Scots pine and causes mortality. Trees with infection grow slower and produce less timber with reduced quality. Despite applied control methods, such as switching tree species to a non-host species, or stump treatment, root and butt rot continues to be a serious forest health problem. Disease resistance breeding is a less-applied control method which has potential to improve tree health. However, neither conifer genotypes with absolute resistance to Heterobasidion sp. nor robust selection markers for resistance breeding have been found. We studied the responses of various Scots pine genotypes to Heterobasidion annosum infection and mechanic damage in drained peatland. Stems and roots of mature naturally regenerated Scots pine trees growing in drained peatland were either artificially infected with H. annosum or wounded and inoculated with sterile inoculum. Untreated trees from the study sites served as controls. Responses of different Scots pine genotypes to pathogen infection as determined by lesion size were recorded from samples harvested four months after inoculation, and least susceptible and highly susceptible genotypes were selected from the study material. Analysis of terpenoids from both least susceptible and highly susceptible pine genotypes by gas chromatography coupled with mass spectrometry indicates that some monoterpenes and sesquiterpenes are differentially induced depending on the susceptibility level. Transcriptomic microarray analysis was therefore conducted with RNA from stems of the least susceptible and highly susceptible Scots pine genotypes. Gene expression data from cDNA microarray were analysed by comparisons between the treatments, and the genotypes with different resistance level. The aim of the study is to highlight transcripts specific to differing levels of susceptibility.

2019-02-01 | GSE66168 | GEO

Pinus sylvestris

Project description:UV-C and CHX response in Scots pine needles of 6-week old seedlings

| PRJNA635551 | ENA