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ABSTRACT: Soybean leaves infected with Phakopsora pachyrhizi for small RNA analysis

PROVIDER: PRJNA855552 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR20226844.fastq.gz	Fastqsanger.gz
	SRR20226845.fastq.gz	Fastqsanger.gz
	SRR20226846.fastq.gz	Fastqsanger.gz
	SRR20226847.fastq.gz	Fastqsanger.gz
	SRR20226848.fastq.gz	Fastqsanger.gz

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Project description:Soybean leaves infected with Phakopsora pachyrhizi

| PRJNA855551 | ENA

Transcription profiling of soybean with respect to Pahakopsora pachyrhizii at V2 growth stage

Project description:A small number of genes (25) expressed differentially with respect to Phakopsora pachyrhizii at V2 growth stage.. We used Affymetrix soybean microarrays to detail the global gene expression underlying host reaction to Phakopsora pachyrhizii and identified differentially expressed genes during this process. Experiment Overall Design: Soybean cv. 5601T plants were grown in greenhouse in six 4-liter pots. Three weeks old plants at V2 growth stage in three out of six pots were inoculated with Phakopsora pachyrhizi and remaining three pots were used as control. Leaf samples from all pots were collected 72 hours after inoculation and RNA was extracted using Tri-Regent. Microarray analysis was performed using Affymetrix platform.

2008-06-15 | E-GEOD-7108 | biostudies-arrayexpress

Expression survey of Rpp1 soybean line PI200492 resistant to P. pachyrhizi

2008-01-31 | GSE8432 | GEO

Transcription profiling of soybean soybean line PI200492 resistant to P. pachyrhizi

Project description:Affymetrix soybean genome arrays were used to identify genes differentially expressed in the immune resistance response at 6, 12, 24, and 48 hours after inoculation with Phakopsora pachyrhizi isolates TW72-1 or HW94-1; soybean + HW94-1 = resistant; soybean + TW72-1 = susceptible Experiment Overall Design: For each of 4 time points (6, 12, 24 and 48 hours post inoculation) a soybean PI200492 plant was inoculated with 2 treatments: HW94-1, or TW72-1. A mock inoculation was performed and sampled at time 0. There were three biological replicates of the entire experiment, for a total of 27 samples. During data analysis two samples failed the quality control metrics and were removed from the analysis, leaving 25 samples in the final data set.

2008-06-16 | E-GEOD-8432 | biostudies-arrayexpress

Molecular characterization of the Rpp4 resistance response

Project description:Experimental design: 2 genotypes: PI- (resistant USDA Plant Introduction (PI459025B) line containing SBR Rpp4 resistance gene) & Cultivar Williams that does not have a known SBR resistance gene 2 treatments: Soybean rust (Phakopsora pachyrhizi) isolate Hawaii 94-1 & mock infection 3 replications 6 time points: 12, 24, 72, 144, 216 and 288 hours after inoculation TOTAL: 72 Affymetrix GeneChip(R) Soybean Genome Arrays Mock treatment: 0.01% Tween 20 Hawaii 94-1 treatment: 500,000 spores per ml in 0.01% Tween 20 ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Steve Whitham. The equivalent experiment is GM37 at PLEXdb.]

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Project description:Experimental design: 1 genotype: PI- (resistant USDA Plant Introduction (PI462312) line containing SBR Rpp3 resistance gene) 3 treatments: Virulent soybean rust (Phakopsora pachyrhizi Tw80-2) challenge, avirulent soybean rust challenge (Hw94-1) & mock infection 3 replications 6 time points: 12, 24, 72, 144, 216 and 288 hours after inoculation TOTAL: 54 Affymetrix GeneChip(R) Soybean Genome Arrays Mock treatment: 0.01% Tween 20 Hawaii 94 treatment: 500,000 spores per ml in 0.01% Tween 20 Taiwan 80-2 treatment: 500,000 spores per ml in 0.01% Tween 20 ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Martijn van de Mortel (for Steve Whitham). The equivalent experiment is GM36 at PLEXdb.]

2011-11-28 | GSE29740 | GEO

Gene expression analysis in soybean with respect to Pahakopsora pachyrhizii at V2 growth stage

Project description:A small number of genes (25) expressed differentially with respect to Phakopsora pachyrhizii at V2 growth stage. We used Affymetrix soybean microarrays to detail the global gene expression underlying host reaction to Phakopsora pachyrhizii and identified differentially expressed genes during this process. Keywords: Infection by pathogen

2007-02-22 | GSE7108 | GEO

Transcriptome‐based analyses of phosphite‐mediated suppression of rust pathogens Puccinia emaculata and Phakopsora pachyrhizi

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Project description:Metatranscriptome of soybean leaves infected with Phakopsora pachyrhizi MG2006 from Stuttgart, Baden-Württemberg, Germany - 8 dpi A

| PRJNA502175 | ENA

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