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Zfp281 and Zfp148 control CD4+ T cell thymic development and Th2 functions

ABSTRACT: Zfp281 and Zfp148 control CD4+ T cell thymic development and Th2 functions

PROVIDER: PRJNA856244 | ENA |

REPOSITORIES: ENA

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Zfp281 and Zfp148 control CD4+ T cell thymic development and Th2 functions

Project description:How CD4+ lineage gene expression is initiated in differentiating thymocytes remains poorly understood. Here, we show that the paralog transcription factors Zfp281 and Zfp148 control both this process and cytokine expression by T helper cell type 2 (TH2) effector cells. Genetic, single-cell, and spatial transcriptomic analyses showed that these factors promote the intrathymic CD4+ T cell differentiation of class II major histocompatibility complex (MHC II)–restricted thymocytes, including expression of the CD4+ lineage–committing factor Thpok. In peripheral T cells, Zfp281 and Zfp148 promoted chromatin opening at and expression of TH2 cytokine genes but not of the TH2 lineage–determining transcription factor Gata3. We found that Zfp281 interacts with Gata3 and is recruited to Gata3 genomic binding sites at loci encoding Thpok and TH2 cytokines. Thus, Zfp148 and Zfp281 collaborate with Gata3 to promote CD4+ T cell development and TH2 cell responses. This SuperSeries is composed of the SubSeries listed below.

2023-11-10 | GSE207572 | GEO

Zfp281 and Zfp148 control CD4+ T cell thymic development and Th2 functions. (ChIP-seq)

Project description:How CD4+-lineage gene expression is initiated in differentiating thymocytes remains poorly understood. Here, we show that the paralog transcription factors Zfp281 and Zfp148 control both this process and cytokine expression by Th2 effector cells. Genetic, single-cell, and spatial transcriptomic analyses show that these factors promote the intrathymic CD4+ T cell differentiation of MHC II-restricted thymocytes, including expression of the CD4+ lineage-committing factor Thpok. In peripheral T cells, Zfp281 and Zfp148 promote chromatin opening at and expression of Th2 cytokine genes, but not of the Th2 lineage determining transcription factor Gata3. Zfp281 interacts with Gata3, and binds Gata3 genomic binding sites at loci encoding Thpok and Th2 cytokines. Thus, Zfp148 and Zfp281 collaborate with Gata3 to promote CD4+ T cell development and Th2 cell responses.

2023-11-13 | GSE207469 | GEO

The transcription factor Zfp281 serves redundantly with Zfp148 to support CD4+ T cell development and functions [Th2 ChIP]

Project description:The transcription factor Gata3 is essential both for CD4+ T cell development in the thymus and for the differentiation of Th2 cells, which mediate responses against extracellular parasites and contribute to asthma and allergies. Here we report that the transcription factor Zfp281, in part redundantly with its paralog Zfp148, cooperates with Gata3 to promote CD4+ T cell development and Th2 cell differentiation. Disruption of both factors in T cells resulted in reduced numbers of spleen CD4+ T cells. Additionally, we show that Zfp281 interacts with Gata3, and that Zfp281 binds to Th2 cytokine loci in Th2 cells. Finally, we found that Zfp148 and Zfp281 promote Th2 cytokine expression. During airway inflammation, Zfp148/281-deficient CD4+ T cells failed to express Th2 cytokines, despite normal expression of Gata3. Altogether our data identify Zfp148 and Zfp281 as essential for enabling Gata3 functions in both CD4+ T cell development and Th2 function.

2023-11-13 | GSE237865 | GEO

The transcription factor Zfp281 serves redundantly with Zfp148 to support CD4+ T cell development and functions [scRNA-seq]

2023-11-13 | GSE206521 | GEO

The transcription factor Zfp281 serves redundantly with Zfp148 to support CD4+ T cell development and functions [Thymus Visium]

2023-11-13 | GSE227332 | GEO

The transcription factor Zfp281 serves redundantly with Zfp148 to support CD4+ T cell development and functions [Papain multiome]

2023-11-13 | GSE227330 | GEO

The transcription factor Zfp281 serves redundantly with Zfp148 to support CD4+ T cell development and functions [Population RNA-seq]

2023-11-13 | GSE206542 | GEO

Zfp281 and Zfp148 control CD4+ T cell thymic development and Th2 functions. (ChIP-seq)

Project description:Zfp281 and Zfp148 control CD4+ T cell thymic development and Th2 functions. (ChIP-seq)

| PRJNA855886 | ENA

The transcription factor Zfp281 serves redundantly with Zfp148 to support CD4+ T cell development and functions [Th2 ChIP]

Project description:The transcription factor Zfp281 serves redundantly with Zfp148 to support CD4+ T cell development and functions [Th2 ChIP]

| PRJNA996959 | ENA

ChIP-seq data for zfp148 and zfp281 chromatin occupancy in hemin-induced human K562 cells

Project description:ChIP-seq was carried for zfp148 and zfp281 in human K562 cells to test the hypothesis that these two transcription factor family members play overlapping roles in erythropoiesis.

2019-09-03 | GSE121133 | GEO

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