Project description:We applied total RNA sequencing of Lactobacillus fermentum strain GR1008 and GR1009 and compare the differentially expressed genes. This study aimed to find out the underlying mechanims for the differential morphology of the two strains.
Project description:Transcription profiling of intestinal tissue from germ free rats mono-colonised with one of two strains of Lactobacillus fermentum; AGR1485 or AGR 1487
Project description:Candida glabrata is a human-associated opportunistic fungal pathogen. It shares its niche with Lactobacillus spp. in the gastrointestinal and vaginal tract. In fact, Lactobacillus species are thought to competitively prevent Candida overgrowth. We investigated the molecular aspects of this antifungal effect by analyzing the interaction of C. glabrata strains with Limosilactobacillus fermentum. From a collection of clinical C. glabrata isolates, we identified strains with different sensitivities to L. fermentum in coculture. We analyzed the variation of their expression pattern to isolate the specific response to L. fermentum. C. glabrata-L. fermentum coculture induced genes associated with ergosterol biosynthesis, weak acid stress, and drug/chemical stress. L. fermentum coculture depleted C. glabrata ergosterol. The reduction of ergosterol was dependent on the Lactobacillus species, even in coculture with different Candida species. We found a similar ergosterol-depleting effect with other lactobacillus strains (Lactobacillus crispatus and Lactobacillus rhamosus) on Candida albicans, Candida tropicalis, and Candida krusei. The addition of ergosterol improved C. glabrata growth in the coculture. Blocking ergosterol synthesis with fluconazole increased the susceptibility against L. fermentum, which was again mitigated by the addition of ergosterol. In accordance, a C. glabrata Derg11 mutant, defective in ergosterol biosynthesis, was highly sensitive to L. fermentum. In conclusion, our analysis indicates an unexpected direct function of ergosterol for C. glabrata proliferation in coculture with L. fermentum.
Project description:Transcription profiling of intestinal tissue from germ free rats mono-colonised with one of two strains of Lactobacillus fermentum; AGR1485 or AGR 1487 Two colour microarray, reference design. 2 treatments (AGR1485 or AGR1487) and 2 tissues analysed (colon or duodenum): Biological replicates: 6
Project description:Lactobacillus fermentum (strain RS2) is a common resident of the human gastrointestinal tract (GIT) and used as potential probiotics. The ability to adapt, colonize and survive in the harsh environment of the GIT, in the presence of gastric juice, is one of the unique properties that helps microflora to transiently harbor the host. In the current study, we used gel free quantification approach coupled with a high-resolution mass spectrometer to analyze the expression pattern of Lactobacillus fermentum RS2 strain under 1.2% bile acid stress condition. The analysis resulted in the identification of 1157 varieties of proteins that are engaged in coping up the efflux of bile effect or protons, by altering the carbohydrate metabolic pathways or through prevention of protein misfolding. The function of downregulated proteins were mostly unknown and the putative functions of the upregulated proteins were categorized as stress response, DNA repair, amino acids metabolism, signal transduction, transcription, translation, and carbohydrate metabolism. The finding suggests that these proteins are involved in bile resistance specific mechanisms as well as processes responsible for adaptation in GIT and showed the association of expression levels of proteins with a precise probiotic trait.
Project description:Transcriptional profiling of Caco-2 cells co-cultured with L. fermentum AGR1487 (isolated from IBD patient), Caco-2 cells co-cultured with L. fermentum AGR1485 (isolated from healthy volunteer), or Caco-2 cells alone (Control).