Ontology highlight
ABSTRACT:
PROVIDER: PRJNA856490 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR20016600.fastq.gz | Fastqsanger.gz | |||
| SRR20016601.fastq.gz | Fastqsanger.gz | |||
| SRR20016602.fastq.gz | Fastqsanger.gz | |||
| SRR20016603.fastq.gz | Fastqsanger.gz | |||
| SRR20016604.fastq.gz | Fastqsanger.gz |
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Project description:The use of blood-based extracellular RNA (exRNA) as clinical biomarker requires the implementation of a validated procedure for sample collection, processing and profiling. So far, no study has systematically addressed the pre-analytical variables affecting transcriptome analysis of exRNAs. In the first phase of the exRNAQC study, we evaluated 10 blood collection tubes, 3 time points between blood draw and downstream processing, and 8 RNA purification methods using the supplier-specified minimum and maximum biofluid input volumes. The impact of these pre-analytics is assessed by deep transcriptome profiling of both small and messenger RNA from healthy donors' plasma or serum. In this second phase, we assessed interactions between blood collection tubes, time points and purification methods.
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Project description:Study to detect possible interactions between blood collection tubes and RNA purification methods on extracellular RNA (exRNA) sequencing. Different combinations of blood collection tubes (3 in total), time intervals between blood draw and downstream processing (immediate (T0), after 4 hours (T4), after 16 hours (T16)), and RNA purification kits (2 in total) were evaluated by applying Small RNA sequencing (Illumina) to exRNA from human healthy donor plasma or serum.
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