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4sU-Seq of human U2OS cells upon acute RBM8A protein depletion

ABSTRACT: 4sU-Seq of human U2OS cells upon acute RBM8A protein depletion

PROVIDER: PRJNA857649 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR20077135.fastq.gz	Fastqsanger.gz
	SRR20077136.fastq.gz	Fastqsanger.gz
	SRR20077137.fastq.gz	Fastqsanger.gz
	SRR20077138.fastq.gz	Fastqsanger.gz
	SRR20077139.fastq.gz	Fastqsanger.gz

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4sU-Seq of human U2OS cells upon acute RBM8A protein depletion

Project description:The impact of acute RBM8A depletion on nascent transcription (using the auxin (IAA)-inducible degron system in conjunction with 4sU-sequencing) was investigated.

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U2OS-ERa, U2OS-ERb, and U2OS-ERab cells treated with 4HT or E2

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Glucocorticoid and androgen receptor profiling (ChIP-seq) in U2OS-GR and U2OS-AR cells upon hormone treatment

Project description:We performed ChIP-seq targeting the glucocorticoid receptor (GR) in the U2OS-GR cell line and the androgen receptor (AR) in the U2OS-AR cell line. The cell lines are derived from U2OS ATTC:HTB-96 and stably transfected with an expression construct for either rat GR or human AR, respectively. The U2OS-GR cells were treated with dexamethasone (1 µM) for 90 minutes. The U2OS-AR cells were treated with R1881 (5 nM) for 4 hours.

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Glucocorticoid and androgen receptor profiling (ChIP-seq) in U2OS-GR and U2OS-AR cells upon hormone treatment

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