Project description:The communacation between the Hepatocellular carcinoma (HCC) and hepatic stellate cells(HSC) is not completely understood. Then a tumor-on-a-chip model was used to evaluate the crosstalk between the HCC cells (HCCLM3) and HSCs (LX2). After 5 days co-culture, we degraded the collagen hydrogel to isolate HCCLM3 cells and LX2 cells for RNA-seq.
Project description:Collagen triple helix repeat containing 1 (CTHRC1) has been found to be up-regulated in many human solid tumors. In this study, we investigated the changes of gene expression by comparing CTHRC1-siRNA and Scramble-siRNA control in hepatocellular carcinoma cell line HCCLM3, which has high expression levels of CTHRC1. Differential gene expression was assessed by Affymetrix microarray experiments for 2 samples: CTHRC1-siRNA-treated HCCLM3 cells and Scramble-siRNA-treated HCCLM3 cells.
Project description:We established GFP/CCDC137-APOBEC1 transfected HCCLM3 cells.Then doxycycline was added to induce the expression of GFP/CCDC137-APOBEC1.We then performed gene expression profiling analysis using RNA obtained from GFP/CCDC137-APOBEC1 overexpressing HCCLM3 cells with or without doxycycline treatment.
Project description:Human myofibroblastic LX2 cells were transfected using a siRNA directed against BNC2 (siBNC2) or a control siRNA (siCTRL) and RNA were processed for microarray analyses.
Project description:Quantitative mass spectrometry (MS)-based proteomics to assess acetylation modification of MYC in HCCLM3 cells with SCARB2 overexpression and analysis of SCARB2 immunoprecipitates in HCCLM3 cells.
Project description:Suberoylanilide hydroxamic acid (SAHA) and valproic acid (VPA) are both histone deacetylases inhibitor (HDACi), and are able to attenuate the activation of hepatic stelllate cells. To explore the underlying molecular mechanisms, we performed gene expression profile analyses of human hepatic stellate cell line LX2 treated with SAHA or VPA for 24 hours. Duplicate experiments were performed: Untreated LX2, SAHA treated LX2 and VPA treated LX2.
Project description:miRNAs are an class of small noncoding RNAs and about 21-25 nucleotides in length. miRNAs inhibit the translation or induce mRNA degradation by binding to the 3’ UTR of target mRNAs and have been identified as the tumor promoters or suppressors regulating the progression of cancers. miR-429, which is a member of an evolutionarily conserved family of miRNAs that includes miR-200b, miR-200a, miR-200c and miR-141, is expressed in various epithelial tissues. Our goal is to search the possible target genes of miR-429 in human liver cancer cell line HCCLM3. We analyzed possible target genes of miR-429 by identifying the shared genes among the down-regulated genes in epithelial cell adhesion molecule (EpCAM) negative HCCLM3 cells which treated with miR-429 mimics and the up-regulated genes in EpCAM positive HCCLM3 cells which treated with Antagomir-429.
Project description:Suberoylanilide hydroxamic acid (SAHA) and valproic acid (VPA ) are both histone deacetylases inhibitor (HDACi), and are able to attenuate the activation of hepatic stelllate cells. To explore the underlying molecular mechanisms, we performed miRNA expression profile analyses of human hepatic stellate cell line LX2 treated with SAHA or VPA for 24 hours. Duplicate experiments were performed: Untreated LX2, SAHA treated LX2 and VPA treated LX2.