Project description:Single cell RNA-seq analysis was performed on sorted population of CD45+/CD34+ HSPCs from cord blood (CB) and mobilized peripheral blood (mPB) donors, after co-culture with vascular niche cells, to uncover differential transcriptional output between HSPC derived from CB and mPB origins.
Project description:Presetting of adaptive hematopoiesis by Fli-1 entails reciprocal niche sensing of Notch/VEGF-A pathways to supersede Spi1-driven dormancy. RNA-seq analysis for WT and Fli-1 KO HSPCs was performed to uncover changes in transcriptional output following Fli-1 deletion.