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ABSTRACT: Transcriptome of Meloidogyne incognita

PROVIDER: PRJNA858760 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR20750000_1.fastq.gz	Fastqsanger.gz
	SRR20750000_2.fastq.gz	Fastqsanger.gz
	SRR20750001_1.fastq.gz	Fastqsanger.gz
	SRR20750001_2.fastq.gz	Fastqsanger.gz
	SRR20750002_1.fastq.gz	Fastqsanger.gz

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Characterization of small RNAs expressed in roots and galls from Arabidopsis thaliana induced by the plant parasitic nematodes Meloidogyne incognita

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RNA-seq of Arabidopsis thaliana Col-0 and opr2-1 mutants over four timepoints and (mock)infection with Meloidogyne incognita

Project description:We compared the gene expression of wild-type Col-0 and a T-DNA mutant SALK_116381C (opr2-1). We either infected or mock-infected the plants with the root knot nematode Meloidogyne incognita and measured the root transcriptome after 0, 1, 4, and 7 days post infection using RNA-seq. The aim of the experiment was to determine whether opr2-1 affected gene expression patterns induced by nematode infection.

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Identification of Novel and Conserved MicroRNAs of Root-knot Nematode Using a Deep-sequencing Approach

Project description:111,556,278 high-quality reads were obtained by a deep-sequencing approach that show an exact match to the genome of Meloidogyne incognita from the library of J2 juveniles of M. incognita (Mi). Based on these Solexa reads, 89 M. incognita microRNA genes were identified, which grouped into 67 non-redundant miRNAs with mature sequences. All of these candidate miRNAs were confirmed by qRT-PCR, and 26 of them could be detected in the library of the galls of cucumber root infected by M. incognita (GC). MiR-100 was found in a cluster with let-7, which is similar with B. malayi, a human parasitic nematode. Based on the results of deep sequencing, the expression of miR-100 was much more abundant than that of let-7, which indicated that they may not be co-expressed. The ortholog of let-7, a key regulator that controls the nematode from L4 to adult in C. elegans, could be frequently sequenced in the GC library, the later stages of development of M. incognita, while it had a relative low expression level in J2, which indicated that let-7 may have a similar role in the development regulation in plant parasitic nematodes. Frequently sequenced microRNAs, including miR-71, miR-100 and miR-124, should play an important role in the growth and proliferation of M. incognita.

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