Transcriptional profiling of IRF8 KO B cell differentiation
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA858849 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR20213600_1.fastq.gz | Fastqsanger.gz | |||
| SRR20213600_2.fastq.gz | Fastqsanger.gz | |||
| SRR20213601_1.fastq.gz | Fastqsanger.gz | |||
| SRR20213601_2.fastq.gz | Fastqsanger.gz | |||
| SRR20213602_1.fastq.gz | Fastqsanger.gz |
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Project description:Transcriptional profiling of IRF8-regulated genes in mouse CD11b-positive primary cells. CD11b-positive cells were isolated from spleens of age-matched wt and IRF8 KO mice, the compared for their gene expression profiles. The objective is to identify genes that are regulated by IRF8 in CD11b-positive primary cells. Two-condition experiments. Wt vs IRF8 KO cells. Biological replicates: 2 replicates for the comparison.
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Project description:Conditional IRF8 KO mice (mice with a conditional allele of Irf8 crossed with CD19-Cre mice) showed increased numbers of both Gene expression data spleen marginal zone (MZ) and Gene expression data spleen follicular (FO) B cells compared to control mice. To evaluate gene expression patterns that distinguished FO or MZ B cells derived from conditional KO and control mice, we used Affymetrix GeneChip® Mouse gene 1.0 ST Array.
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