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Transcriptomic analysis of the bone reveals the mechanism of Desertliving Cistanche herb in ovariectomized rats of postmenopausal osteoporosis

ABSTRACT: Transcriptomic analysis of the bone reveals the mechanism of Desertliving Cistanche herb in ovariectomized rats of postmenopausal osteoporosis

PROVIDER: PRJNA859135 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR20225959_1.fastq.gz	Fastqsanger.gz
	SRR20225959_2.fastq.gz	Fastqsanger.gz
	SRR20225960_1.fastq.gz	Fastqsanger.gz
	SRR20225960_2.fastq.gz	Fastqsanger.gz
	SRR20225961_1.fastq.gz	Fastqsanger.gz

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Transcriptomic analysis of the bone reveals the mechanism of Desertliving Cistanche herb in ovariectomized rats of postmenopausal osteoporosis

Project description:To investigate the function of Desertliving Cistanche herb in the postmenopausal osteoporosis, we established ovariectomized rats in which both ovaried were removed.We performed gene expression profiling alalysisi using date obtained from RNA-seq of ovariectomized rats of postmenopausal osteoporosis.

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Identification of exosomal circRNAs as potential biomarkers for postmenopausal osteoporosis

Project description:Background: Osteoporosis is one of the most common chronic diseases around the world, especially in postmenopausal women. Circular RNAs (circRNAs) are emerging as important regulators in human disease. The aim of the present study was to analyze circRNA expression profiles in osteoporosis and to explore the clinical significance. Methods: The serum samples were collected from osteoporosis patients and healthy people. The circRNA array was performed to screen for differentially expressed circRNAs. The mRNA expression levels of target genes were analyzed by quantitative real-time PCR (qRT-PCR). Results: Hsa_circ_0006859 was identified as one of the most upregulated circRNAs in the microarray analysis. Hsa_circ_0006859 in exosome was upregulated in osteoporosis patients compared with healthy controls. Hsa_circ_0006859 could differentiate osteopenia or osteoporosis patients from healthy controls with high sensitivity and specificity. Conclusion: Exosomal hsa_circ_0006859 is a potential biomarker for postmenopausal osteoporosis.

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