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Next Generation Sequencing Facilitates Quantitative Analysis of SKOV3 cells overexpression of TNFAIP8L2

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of SKOV3 cells overexpression of TNFAIP8L2

PROVIDER: PRJNA859663 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR20280559_1.fastq.gz	Fastqsanger.gz
	SRR20280559_2.fastq.gz	Fastqsanger.gz
	SRR20280560_1.fastq.gz	Fastqsanger.gz
	SRR20280560_2.fastq.gz	Fastqsanger.gz
	SRR20280561_1.fastq.gz	Fastqsanger.gz

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Next Generation Sequencing Facilitates Quantitative Analysis of SKOV3 cells overexpression of TNFAIP8L2

Project description:Purpose: Next generation sequencing (NGS) has revolutionized system-based analysis of cellular pathways. The goals of this study are to compare NGS-derived transcriptome profiling of human ovarian cancer cell, SKOV3 cells after endogenous overexpression of TNFAIP8L2 (TIPE2) . Methods: We constructed TIPE2 overexpression SKOV3 cell lines and control vector cell lines with lentivirus. Then the NGS based deep sequencing analysis was generated in triplicate to analyze the critical downstream targets after TIPE2 overexpression using ILLumina GAIIx. Results: The differentially expressed genes were identified using an optimized data analysis workflow, and we identified 49 upregulated and 28 downregulated genes in the SKOV3/TIPE2 group compared with the vector group using a |log2Foldchange|﹥0 and p < 0.05. Conclusions: Our study represents the first detailed analysis of transcriptomes in ovarian cancer cell line after overexpression of TIPE2 by RNA-seq technology.

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