Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Lewis Lung Carcinoma (LLC) cells in the lung model express lung-specific genes compared to subcutaneous engraftment cells.

ABSTRACT: Lewis Lung Carcinoma (LLC) cells in the lung model express lung-specific genes compared to subcutaneous engraftment cells.

PROVIDER: PRJNA859705 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Microarray analysis of T-cell death-associated gene 8 (TDAG8)-mediated gene expression in mouse Lewis lung carcinoma (LLC) cells.

Project description:We previously identified an extracellular pH-sensing G protein-coupled receptor T cell death associated gene 8 (TDAG8) as an extracellular pH sensor of tumor cells that promotes cell growth/survival in vitro and tumor development in vivo. To determine genes regulated by TDAG8 in vitro, mouse Lewis lung carcinoma (LLC) cells stably expressing TDAG8 (TDAG8-LLC cells) and control vector (control LLC cells) were cultured under acidic conditions. Transcriptome microarray analysis using Affymetrix GeneChip Mouse Genome 430 2.0 Arrays was performed with total RNA prepared from these cells. To determine genes regulated by TDAG8 in vitro, mouse Lewis lung carcinoma (LLC) cells stably expressing TDAG8 (TDAG8-LLC cells) and control vector (control LLC cells) were cultured under acidic conditions. Transcriptome microarray analysis using Affymetrix GeneChip Mouse Genome 430 2.0 Arrays was performed with a mixture of equal amounts of total RNA samples from four independent cell cultures.

2012-11-01 | E-GEOD-31776 | biostudies-arrayexpress

Lewis lung carcinoma cells and TDAG8 overexpression

Project description:We observed the effects of TDAG8-overexpression in Lewis lung carcinoma (LLC) cells on the gene expression pattern.

2010-09-06 | GSE18244 | GEO

Microarray analysis of T-cell death-associated gene 8 (TDAG8)-mediated gene expression in mouse Lewis lung carcinoma (LLC) cells.

2012-11-01 | GSE31776 | GEO

Characterization of tumor microenvironment using single cell RNA sequencing in Lewis Lung Carcinoma cell transplantation mouse model

Project description:The tumor microenvironment holds significant importance in cancer development and progression. Research conducted using mouse models of tumor cell transplantation can enhance our comprehension of the tissue microenvironment that allows tumor growth at an early stage. Among these models, those featuring the Lewis Lung Carcinoma (LLC) cell line are noteworthy due to their low immunogenic features. Since numerous cancer patients do not respond effectively to current immunotherapy, it is imperative to investigate models with low immunogenic features. Consequently, this study employed single-cell RNA sequencing on an orthotopic lung cancer mouse model utilizing the LLC cell line to profile the characteristics of diverse cell types that emerge in the lung during tumor engraftment. The lung microenvironment exhibited discernible transformations, including fibrogenic gene expression, increased cell-to-cell and cell-to-extracellular matrix adhesion, and reduced infiltration of T cells compared to lungs without tumor engraftment. In conclusion, these findings reveal cellular and molecular alterations that can be targeted for tumor prevention and treatment for low-immunogenic tumors.

2024-10-28 | GSE246710 | GEO

Lewis lung carcinoma cells and TDAG8 overexpression

Project description:We observed the effects of TDAG8-overexpression in Lewis lung carcinoma (LLC) cells on the gene expression pattern. TDAG8-overexpressing or vector-transfected control LLC cells (5 × e5 in 200 µL of HBSS) were injected into the lateral tail veins of 6–8-week-old C57BL/6 mice. Lungs were removed on day 19 post-injection, and total RNA was extracted.

2010-09-06 | E-GEOD-18244 | biostudies-arrayexpress

Whole transcriptome of Lewis lung carcinoma cells of Mus musculus

Project description:Purpose: To study the alteration of whole transcriptome of Lewis lung carcinoma (LLC) cells after the decreasing of malignant properties of tumor by treatment of tumor-bearing mice with RNase A. Methods: Whole transcriptome profile of Lewis lung carcinoma before and after RNase A treatment were generated by deep sequencing using SOLiD 5.5. The sequence reads were mapped by Bioscope 1.3 software, differential expression was evaluated by Cufflinks v.2.0.1 package. Results: Difference in expression was found for 966 genes. Conclusions: Our study represents the first detailed analysis of alteration of transcriptome of Lewis lung carcinoma after the decrease of malignant prtoperties of the tumor (proliferation and invasion) by RNase A.

2014-12-03 | GSE63758 | GEO

Lewis Lung Carcinoma (LLC) cells in the lung model express lung-specific genes compared to subcutaneous engraftment cells.

Project description:We revealed that tumor cells in the bleomycin-injured lungs (BLM lungs) are differently influenced by their unique lung fibrogenic microenvironment compared to those in subcutaneous tumor models in vivo or in 2-dimensional monolayer culture in vitro.

2022-07-19 | GSE208373 | GEO

Whole transcriptome of Lewis lung carcinoma cells of Mus musculus

2014-12-03 | E-GEOD-63758 | biostudies-arrayexpress

Transcriptomic computational analysis of mastic oil-treated Lewis lung carcinomas

Project description:Mastic oil from Pistacia lentiscus variation chia, a blend of bioactive terpenes with recognized medicinal properties, has been recently shown to exert anti-tumor activity. Lewis lung carcinoma (LLC) cells are mastic oil-susceptible cells and were used in this work to study the effects of mastic oil at the transcriptomic level.

2009-06-01 | GSE15287 | GEO

Lewis Lung Carcinoma UROD

Project description:Lewis Lung Carcinoma UROD

| PRJNA578483 | ENA