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ABSTRACT: Bacterial community of sweet sorghum silage

PROVIDER: PRJNA860017 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR20461753_1.fastq.gz	Fastqsanger.gz
	SRR20461753_2.fastq.gz	Fastqsanger.gz
	SRR20461754_1.fastq.gz	Fastqsanger.gz
	SRR20461754_2.fastq.gz	Fastqsanger.gz
	SRR20461755_1.fastq.gz	Fastqsanger.gz

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Project description:sweet sorghum silage

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Identification of non-coding RNA in sweet sorghum under salt stress

Project description:Salt stress has become one of the main abiotic stress factors restricting agricultural production worldwide. Sweet sorghum is an important salt and drought tolerant feed and energy crop. Its salt tolerance mechanism has not been widely studied. With the development of transcriptome sequencing technology, it is possible to study the molecular mechanism of sweet sorghum salt tolerance. The purpose of this study was to further reveal the potential salt-tolerant molecular mechanisms of sweet sorghum through high-throughput sequencing analysis of the transcriptome. Finally, through high-throughput sequencing, we read approximately 54.4G of raw base and 53.7G of clean base in total, and used FastQC to assign a quality score (Q) to each base in the read using a similar phred algorithm, Analysis shows that the data is highly credible. We conclude that RNA-based transcriptome characterization will accelerate the study of genetics and molecular biology of sweet sorghum salt tolerance mechanisms and provide a framework for this.

2020-03-19 | GSE145748 | GEO

Identification of N6-methyladenosine (m6A) modification in sweet sorghum under salt stress

Project description:The N6-methyladenosine (m6A) modification is the most common internal post-transcriptional modification, with important regulatory effects on RNA export, splicing, stability，and translation. However, the effects of m6A modifications on the resistance of sweet sorghum to salt stress remain unclear. In this study, we mapped the m6A modifications in two sorghum inbred lines (salt-tolerant M-81E and salt-sensitive Roma) that differ regarding salt tolerance. Dynamic changes to m6A modifications in sweet sorghum were identified in response to salt stress. Our data suggest that the differences in the m6A modifications between salt-tolerant and salt-sensitive sweet sorghum might contribute to the diversity in salt tolerance.

2022-05-02 | GSE146936 | GEO

Sorghum gene expression using Agilent custom 4x44K Microarray

Project description:As no commercial array is available for sorghum microarray analysis, we designed an array based on the annotation of Sbi1.4 gene set and the available 209,835 sorghum ESTs from the NCBI EST database. The array will be used for investigating the expression divergence between grain and sweet sorghum lines under normal and sucrose treatments The expression analysis was carried out using 14-day old whole seedlings from both grain and sweet sorghum lines. Three samples from sucrose treatment (0h, 2h and 6h) for each line were collected for the analysis . Two biological replicates were carried out for both control and sucrose treatments, resulting in a dataset of 12 microarrays.

2012-04-07 | E-GEOD-36689 | biostudies-arrayexpress

small RNAs sequencing in sweet sorghum under cadmium stress

Project description:Four small RNA libraries from two contrasting sweet sorghum genotypes were sequenced. In this study, One hundred and ninety-five conserved miRNAs belonging to 56 families and 25 putative novel miRNAs from 28 precursors were identified, among which 38 conserved and 24 novel miRNAs were differentially expressed under Cd stress and/or between H18 and L69. Two groups of them: miR169p/q-nov_23 and miR408 were further focused through the coexpression analysis and might be involved in Cd transport, cytoskeleton activity and cell wall construction by regulating their targets. This study presents new insights into the regulatory roles of miRNAs in Cd accumulation and tolerance in sweet sorghum and will help to develop high-Cd accumulation or high Cd-resistant germplasm of sweet sorghum through molecular breeding and/or genetic engineering approaches.

2023-10-12 | GSE145617 | GEO

Sorghum gene expression using Agilent custom 4x44K Microarray

2012-04-08 | GSE36689 | GEO

Stem tranascriptome of sweet sorghum variety ICSA38

Project description:We report transcriptome profiling from three developing stages of middle internode in sweet sorghum. Samples were harvested at Booting, milky stages and physiological maturity.

2023-12-31 | GSE153690 | GEO

Effects and mechanism of Neolamarckia cadamba essential oil on the aerobic stability of whole-plant corn silage

Project description:The purpose of this study was to explore the mechanism of aerobic decay of whole-plant corn silage and the effect of Neolamarckia cadamba essential oil on aerobic stability of whole-plant corn silage. Firstly, the dynamic changes of temperature, microbial community and metabolite content after aerobic exposure of whole-plant corn silage were determined, and the main microbial species and mechanism leading to aerobic spoilage of whole-plant corn silage were analyzed. The N. cadamba essential oil was extracted from fresh N. cadamba leaves by steam distillation, and the minimal inhibitory concentration, antibacterial stability and bacteriostatic mechanism of N. cadamba essential oil against undesirable microorganisms in whole-plant corn silage were determined. According to the minimum inhibitory concentration of N. cadamba essential oil on undesirable microorganisms in silage, N. cadamba essential oil was added to whole-plant corn silage to explore the effect of N. cadamba essential oil on the aerobic stability of whole-plant corn silage.

2023-09-13 | GSE241081 | GEO

Stem transcriptome of sweet sorghum hybrid CSH22SS during development and in response to water limiting conditions.

Project description:We report transcriptome profiling from three developing stages of middle internode in Hybrid sweet sorghum. Samples were harvested at Booting, milky stages and physiological maturity.

2023-12-31 | GSE153418 | GEO

Phylogenomic Analysis of R2R3 MYB Transcription Factors in Sorghum and their Role in Conditioning Biofuel Syndrome

Project description:We report transcriptome profiling from three developing stages of middle internode in sweet sorghum. Sequencing of 6 libraries (two replicates from each stage) each stage yielded approximately 80 million reads.

2022-04-30 | GSE148558 | GEO

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