Project description:We used RNA-seq to determine transcriptional profiles of whole guts or IPCs isolated from guts infected with wild type or type VI secretion system deficient Vibrio cholerae. We found significant differences between guts and progenitor cells infected wild type or type VI secretion system deficient Vibrio cholerae.
Project description:Pseudomonas syringae, a highly destructive plant bacterial pathogen causing severe disease and significant yield losses in agriculture globally, has complex regulatory systems involving many transcriptional factors (TFs). Although the LysR-type transcriptional regulator (LTTR) family proteins are a well-known group of TFs involved in diverse physiological functions, the roles of LTTRs in P. syringae are still largely unknown. In this study, we characterised a LysR-type TF, PSPPH4638, which is a virulence and metabolism regulator (VimR) with multiple functions. Genome-wide identification of VimR using chromatin immunoprecipitation sequencing revealed 1,032 binding sites in the genome, of which 85% were in intergenic regions. Transcriptomic analysis showed altered expression of 454 and 82 genes in response to vimR deletion in King’s B medium (KB) and minimal medium, respectively. Conjoint analysis showed that 99 genes were directly affected by VimR in KB. VimR was identified as a repressor of the type III secretion system, oxidative stress response and key metabolic pathways such as the tricarboxylic acid cycle. In addition, we found that VimR was positively involved in the type VI secretion system and alanine, aspartate and glutamate metabolism. Taken together, our findings identified a new TF VimR and revealed its important functions in P. syringae. This is the first time to describe a transcription factor that regulates both type III secretion system and type VI secretion system and multiple metabolic pathways in Pseudomonas syringae.
Project description:TraDIS study to identify novel immunity proteins and their effector proteins associated with the Type VI secretion system (T6SS) in Pseudomonas aeruginosaThese data are part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/