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ABSTRACT: RNA-Seq of Spodoptera frugiperda hemocytes and fat bodies following viral infection

PROVIDER: PRJNA861097 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR20387200_1.fastq.gz	Fastqsanger.gz
	SRR20387200_2.fastq.gz	Fastqsanger.gz
	SRR20387201_1.fastq.gz	Fastqsanger.gz
	SRR20387201_2.fastq.gz	Fastqsanger.gz
	SRR20387202_1.fastq.gz	Fastqsanger.gz

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Effect of HdIV or MdBV injection on the Spodoptera frugiperda hemocyte transcriptome

Project description:Comparison of the effects of 2 polydnaviruses (HdIV vs MdBV) injection on L5 Spodoptera frugiperda larvae hemocytes transcriptome.

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Effect of HdIV or MdBV injection on the Spodoptera frugiperda hemocyte and fat body transcriptomes

Project description:This SuperSeries is composed of the following subset Series: GSE16775: Effect of HdIV or MdBV injection on the Spodoptera frugiperda hemocyte transcriptome GSE16776: Effect of HdIV or MdBV injection on the Spodoptera frugiperda fat body transcriptome Refer to individual Series

2011-04-05 | E-GEOD-17293 | biostudies-arrayexpress

RNA-seq of three Spodoptera frugiperda tissues (Midgut, Fat body, Hemocytes), at 2 time-points (8h and 15h) after infestation by entomopathogenic nematodes Steinernema carpocapsae/Xenorhabdus nematophila

Project description:Nematodes such as Steinernema carpocapsae are used as organic pesticides because of their ability to prey on live insects. They do so thanks to their symbiotic bacteria, that they release within the hemocoel of insects. In this study we wanted to study how Spodoptera frugiperda, a Lepidoptera pest of crops becoming invasive around the world, defend themselves against the nematodes. We infested S. frugiperda larvae with nematodes and dissected three tissues: the midgut, the fat body and the hemocytes at two time-points: 8 h and 15 h after infestation. We performed single-end RNA-seq on these samples to study the tissue specificity of S. fru response, as well as its dynamic.

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Effect of HdIV or MdBV injection on the Spodoptera frugiperda hemocyte transcriptome

Project description:Comparison of the effects of 2 polydnaviruses (HdIV vs MdBV) injection on L5 Spodoptera frugiperda larvae hemocytes transcriptome. The experimental design is as follows: 3 biological replicates with dye swap. Each treatment (HdIV or MdBv) is compared to control treatment (PBS).

2011-04-05 | E-GEOD-16775 | biostudies-arrayexpress

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Project description:System level knowledge of host alterations is crucial to elucidate the molecular events of viral pathogenesis and to develop strategies to block viral establishment and amplification. Here, we applied quantitative proteomics approach to study global proteome changes in the host; Spodoptera frugiperda upon infection by a baculovirus, Spodoptera litura NPV at two stages i.e. 12h and 72h post infection. At 12hpi, >95% of host proteins remained stable, however at 72hpi, 52% host proteins exhibited downregulation of 2-fold or more. Functional analysis revealed significant upregulation of transposition and proteasomal machinery while translation, transcription, protein export and oxidative phosphorylation pathways were adversely affected. An assessment of perturbed proteome after viral infection and viral miRNAs expression led to the identification of 117 genes that are potential targets of 10 viral miRNAs. Using miRNA mimics, we confirmed the down regulation of 9 host genes. The results comprehensively show dynamics of host responses after viral infection.

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