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Uterine infection-induced transcriptomic changes in the bovine endometrium

ABSTRACT: Uterine infection-induced transcriptomic changes in the bovine endometrium

PROVIDER: PRJNA862118 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR20631500_1.fastq.gz	Fastqsanger.gz
	SRR20631500_2.fastq.gz	Fastqsanger.gz
	SRR20631501_1.fastq.gz	Fastqsanger.gz
	SRR20631501_2.fastq.gz	Fastqsanger.gz
	SRR20631502_1.fastq.gz	Fastqsanger.gz

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Uterine infection-induced transcriptomic changes in the bovine endometrium

Project description:Purpose: Perform RNA-seq study on infectious bovine endometrial tissues to reveal important genes and biological pathways regulating uterine physiology following uterine infections Methods:RNA sequencings were done using Illumina platform. Single-end reads in the FASTQ format were explored using FastQC, low-quality reads were trimmed from both 3’ and 5’ ends until a base pair of Phred quality score of 30 (99.9% accurate) or greater was found, reads having a mean quality score less than 30 and length below 30 nucleotides were filtered out. Cleaned reads were aligned against the bovine reference genome (Bos_taurus.ARS-UCD1.2) using HiSAT2. The resulting SAM files were sorted, converted to BAM files using SAMtools. Read counts mapped to bovine gene models were generated using htseq-count script from HTSeq package. Bioconductor DESeq2 was used to get the differentially expressed genes among infectious vs normal uterine tract groups Conclusions: The study demonstrated that uterine infections altered several genes and pathways related to inflammatory response, immune response, uterine physiology, uterine enviroment and fertility in the intercaruncular region of bovine endometrium.

2022-07-28 | GSE209700 | GEO

TRANSCRIPTOMIC ANALYSIS OF BOVINE ENDOMETRIUM WITH SHORT UTERINE TRACT LEADING TO INFERTILITY

Project description:Purpose: Perform RNA-seq study on short bovine endometrial tissues to reveal important genes and biological pathways related to uterine development and physiology Methods:RNA sequencings were done using Illumina platform. Single-end reads in the FASTQ format were explored using FastQC, low-quality reads were trimmed from both 3’ and 5’ ends until a base pair of Phred quality score of 30 (99.9% accurate) or greater was found, reads having a mean quality score less than 30 and length below 30 nucleotides were filtered out. Cleaned reads were aligned against the bovine reference genome (Bos_taurus.ARS-UCD1.2) using HiSAT2. The resulting SAM files were sorted, converted to BAM files using SAMtools. Read counts mapped to bovine gene models were generated using htseq-count script from HTSeq package. Bioconductor DESeq2 was used to get the differentially expressed genes among short vs normal uterine tract groups Conclusions: Heifers with short uterine tract had significantly decreased endometrial layers, uterine glands, and altered transcriptomic profiles. The decrease in uterine glands probably resulted in lower uterine secretions necessary to support embryo growth and development. As a result, heifers with short uteri were infertile even when they were bred by fertile bulls.

2022-07-28 | GSE209699 | GEO

Uterine infection alters bovine endometrium, oviduct and granulosa transcriptome three months later

Project description:Uterine infection alters bovine endometrium, oviduct and granulosa transcriptome three months later

| PRJNA589857 | ENA

TRANSCRIPTOMIC ANALYSIS OF BOVINE ENDOMETRIUM WITH SHORT UTERINE TRACT LEADING TO INFERTILITY

Project description:TRANSCRIPTOMIC ANALYSIS OF BOVINE ENDOMETRIUM WITH SHORT UTERINE TRACT LEADING TO INFERTILITY

| PRJNA862119 | ENA

Temporal changes of gene expression in bovine endometrium after parturition

Project description:To clarify the regenerative mechanism of endometrium after parturition in cows, mRNA expression profiles in bovine endometrium were investigated during postpartum period. after PVP-I treatment in cows. The differentially expressed genes in the endometrium between postpartum days 49-52 and days 99-101 were 23 genes, and they were much lower than those before postpartum days 49-52. This result suggests that endometrial regeneration after parturition is completely accomplished until postpartum days 49-52.

2021-01-16 | GSE164924 | GEO

Uterine infection alters bovine endometrium, oviduct and granulosa transcriptome three months later

Project description:Here we tested the hypothesis that intrauterine infusion of pathogenic bacteria leads to changes in the transcriptome of the reproductive tract in dairy cattle three months later. We used virgin Holstein heifers to avoid the confounding effects of periparturient problems, metabolic stress and lactation. Animals were infused intrauterine with endometrial pathogenic bacteria Escherichia coli and Trueperella pyogenes to induce clinical endometritis (n = 4) and compared with control animals (n = 6). Three months after infusion, the caruncular and intercaruncular endometrium, isthmus and ampulla of the oviduct, and granulosa cells from dominant ovarian follicles were profiled by RNA-sequencing. Compared with control, bacterial infusion altered the transcriptome of all the tissues. Most differentially expressed genes were tissue-specific, with 109 differentially expressed genes unique to the caruncular endometrium, 57 in the intercaruncular endometrium, 65 in the isthmus, 298 in the ampulla, and 83 in granulosa cells. Surprisingly, despite infusing the bacteria into the uterus, the granulosa cells had more predicted upstream regulators of differentially expressed genes than all the other tissues combined. In conclusion, there was evidence of long-term changes to the transcriptome of the endometrium, oviduct and even the granulosa cells after intrauterine infusion of pathogenic bacteria, which implies that all these tissues contribute to the infertility that persists after endometritis.

2019-11-30 | GSE140469 | GEO

Expression data from bovine conceptus during peri-implantation period

Project description:Conceptus implantation to the uterine endometrium is required for pregnancy establishment, during which non-invasive trophoblasts attach and adhere to the uterine endometrium or invasive trophoblasts invade into the uterine stroma, followed by placental formation in most mammalian species. During peri-implantation period, conceptuses must communicate with the uterine endometrium if they are to survive and proceed to attachment to the uterine epithelium. Despite numerous studies performed on the bovine species, molecular mechanisms associated with their attachment processes, particularly the initial attachment to the endometrial epithelium, have not been well characterized.

2017-12-01 | GSE99654 | GEO

Influence of bovine virus diarrhoea virus on the transcriptome profile of bovine endometrium in response to bacterial lipopolysaccharide.

Project description:Infection with non-cytopathic bovine viral diarrhea virus (ncpBVDV) is associated with uterine disease and infertility. This study investigated the influence of ncpBVDV on immune functions of the bovine endometrium by testing the response to bacterial lipopolysaccharide (LPS) at the level of whole-transcriptomic gene expression. Analysis showed that approximately 30% of the 1,006 genes altered by LPS are involved in immune response. Many innate immune genes that typically respond to LPS were inhibited by ncpBVDV including those involved in pathogen recognition, inflammation, interferon response, chemokines, tissue remodeling, cell migration and cell death/survival. Infection with ncpBVDV can thus compromise immune function and pregnancy recognition thereby potentially predisposing infected cows to postpartum bacterial endometritis and reduced fertility.

2015-08-05 | GSE71696 | GEO

Effect of intrauterine MGO administration on bovine endometrium

Project description:To clarify the effect of intrauterine methylglyoxal (MGO) administration on the endometrium, mRNA expression profiles of bovine endometrium were investigated. Hierarchical cluster analysis with the expression levels of all genes was divided these cows into two clusters. First cluster was composed of control cows, second cluster contained MGO (5 mM) treated cows.

2021-01-19 | GSE165039 | GEO

Transcriptomic Profile of the Bovine Endosalpinx & Endometrium to Identity Putative Embryokines

Project description:Although the oviduct and uterus regulate early embryonic development, understanding of the specific maternally-derived molecules that signal to the embryo is incomplete. Objectives of this study were to characterize expression of genes encoding for growth factors, cytokines, and other cell-signaling ligands in the endosalpinx and endometrium of the bovine female and analyze spatial changes in gene expression throughout the reproductive tract.

2023-09-12 | GSE230154 | GEO

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