Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR20643200_1.fastq.gz | Fastqsanger.gz | |||
| SRR20643200_2.fastq.gz | Fastqsanger.gz | |||
| SRR20643201_1.fastq.gz | Fastqsanger.gz | |||
| SRR20643201_2.fastq.gz | Fastqsanger.gz | |||
| SRR20643202_1.fastq.gz | Fastqsanger.gz |
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Similar Datasets
Project description:succinate (SUC), as a feed additive, can regulate muscle fiber structure and fat deposition, and has a positive effect on meat quality. In this study, 30 male Tan sheep lambs were selected to investigate the effects of SUC in diet on Tan sheep production performance, lamb quality, muscle fiber structure, liver and longissimus' muscle entities (LT) transcriptomes. Different levels of SUC were fed (0%, 1.0%, 2.0%, respectively) and the experiment lasted for 60 days. Compared with CON group, 2% SUC group significantly increased average daily gain from 1 to 15 days and 46 to 60 days during the experiment period, and significantly increased dry matter intake from 1 to 15 days, 16 to 30 days and 46 to 60 days during the experiment period (P<0.05). Both 1% SUC group and 2% SUC group could decrease feed to gain ratio and increase carcass weight (P<0.05). The slaughter rate of 2% SUC group was significantly increased (P=0.012). In both 1% SUC and 2% SUC groups, shear force and cooking loss were decreased, intramuscular fat was increased, and a* of LT muscle was increased (P=0.036). In addition, the muscle fiber structure of Tan sheep LT muscle in 1% SUC group and 2% SUC group was changed, the diameter of muscle fiber was decreased, the cross-sectional area of type Ⅰ and type Ⅱa muscle fiber was decreased, and the density of type Ⅰ muscle fiber was increased (P<0.05). Transcriptomic results showed that the addition of SUC can change the expression of muscle development and muscle fiber type transformation genes. Differential genes are mainly involved in various pathways related to lipid metabolism, energy metabolism and muscle development, improve muscle glucose uptake capacity, and improve meat quality in circadian rhythm. In conclusion, SUC can regulate the nutritional metabolism of Tan Sheep, change the muscle fiber structure of Tan Sheep, and improve the production performance and meat quality. Our results show that adding 1% SUC to the Tan sheep diet is productively more beneficial.
2025-12-26 | GSE285317 | GEO
Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of miRNAs in goat muscle tissue.We identified 1120 miRNAs in fetus and lamb muscle tissue; 895 were known miRNAs and 313 were novel miRNAs. According to analysis, 192 miRNAs were up-regulated in fetus samples compared to lamb samples, while 121 miRNAs were down-regulated.To confirmed stage-specific differences in the abundance of certain miRNAs, we focused on the most up-regulated miRNA and found to be predominantly expressed in lamb muscle tissue, suggesting a potential role in muscle development.It has become a molecular marker for breeding programs of mutton production.
2025-02-01 | GSE196948 | GEO
Project description:To investigate the impact of adding succinate to the diet on the production performance, meat quality, muscle fiber characteristics, and transcriptome of the longissimus dorsi muscle in Tan sheep, 36 Tan sheep were selected and fed with different levels of succinate (0%, 0.5%, 1.0%, 2.0%) for a 60-day trial period. Overall, compared to the control group, the addition of succinate to the diet improved the production performance, slaughter performance, and meat quality of Tan sheep. It significantly increased dry matter intake, carcass weight, eye muscle area, and the GR value while significantly reducing the shear force and cooking loss of the longissimus dorsi muscle (p<0.05). Furthermore, the addition of succinate to the diet altered the muscle fiber characteristics of the longissimus dorsi muscle in Tan sheep, significantly increasing the fiber diameter and cross-sectional area of type I and type IIa muscle fibers (p<0.05). The addition of 1.0% succinate to the diet altered the transcriptome of the longissimus dorsi muscle in Tan sheep, with 741 differentially expressed genes identified compared to the control group. These differentially expressed genes were involved in various pathways related to lipid metabolism, energy metabolism, and muscle development, such as insulin secretion, insulin resistance, cAMP signaling pathway, PI3K-Akt signaling pathway, and FoxO signaling, among others. In summary, succinate plays a crucial role in regulating energy metabolism, protein deposition, and glucose and lipid metabolism homeostasis in Tan sheep through insulin signaling pathways and the interaction of muscle cell factors. By modulating the expression of relevant genes, succinate improves the muscle fiber characteristics of Tan sheep, thereby enhancing production performance and meat quality.
2023-11-26 | GSE248353 | GEO
Project description:CDK4/hTERT immortalized ovine satellite cells were established from primary lamb latissimus dorsi muscle and comprehensively characterized using RNA-seq across three populations: primary lamb satellite cells (PLM), early-passage immortalized cells (EPILM, passage 5), and late-passage immortalized cells (LPILM, passage 30). Differential gene expression and functional enrichment analyses were performed to assess immortalization-driven molecular reprogramming, myogenic competence, safety, and suitability for cultivated meat applications.
2026-09-03 | GSE336565 | GEO
Project description:<p>This study investigated age-associated changes in the gut-muscle axis and their potential contribution to meat quality in Tan sheep (Ovis aries). Twelve Tan sheep were divided into two age groups, including six 3-month-old animals (T3) and six 6-month-old animals (T6). Untargeted LC-MS metabolomics was performed to characterize serum metabolic profiles, and untargeted lipidomics was used to investigate lipid remodeling in longissimus dorsi muscle. In parallel, rumen microbial community profiles were analyzed to explore potential associations between microbiota-derived metabolites, host metabolism, and muscle lipid composition. Particular attention was paid to L-arginine- and serotonin-related metabolic signaling and their associations with muscle lipid remodeling and meat quality. The dataset provides mass spectrometry-based metabolomic and lipidomic profiles for investigating age-related metabolic regulation and gut-muscle interactions in Tan sheep.</p>
2026-09-03 | MTBLS15419 | MetaboLights
Project description:To explore functional lncRNAs during sheep muscle growth, we systematically investigated lncRNAs using strand-specific Ribo-Zero RNA Sequencing at three key developmental stages of Hu sheep (110-day fetus, 5-day-old lamb and 2-year-old adult)
2017-07-21 | GSE101669 | GEO
Project description:Purpose: To explore the mechanism of how Tan I inhibits malignant hematopoiesis at the gene expression level Methods: NB4 cells treated with DMSO or Tan I 10 μM were collected for RNA sequencing Results: We found that 376 genes were differentially expressed between DMSO and Tan I treatment in the NB4 cells (p value <=0.05, |log2 fold change| >= 0.25) Conclusions: We identified MMP9 and ABCG2 as two possible downstream genes of Tan I’s effects on EZH2
2020-08-04 | GSE155572 | GEO
Project description:Purpose: To explore the mechanism of how Tan I inhibits malignant hematopoiesis at the gene expression level Methods: 72 hpf Tg(c-mybhyper: GFP) zebrafish embryos treated with DMSO or Tan I 60 μM were collected for RNA sequencing Results: We found that 1882 genes were differentially expressed between DMSO and Tan I treatment in c-mybhyper fish embryos (p value <=0.05, |log2 fold change| >= 0.25) Conclusions: We identified MMP9 and ABCG2 as two possible downstream genes of Tan I’s effects on EZH2
2020-08-04 | GSE155573 | GEO
Project description:Thermal ablation offers minimally invasive treatment options for hepatocellular carcinoma (HCC) therapy. However, local recurrence due to sublethal temperatures enhances tumor cell survival. This study aims to investigate the tumor-promoting effects of hyperthermia on HCC cells, the role of tanshinone IIA (Tan IIA) in mitigating these effects, and the underlying mechanisms involved. We observed that temperature at 44 °C increased the aggressiveness of HCC cells, and Tan IIA inhibited cell viability and cell invasion, and induced cell cycle arrest and apoptosis of heat-pretreated HCC cells. ALDH7A1 was identified as a target of Tan IIA, and its altered expression resulted in dysregulation of cell viability, invasion, apoptosis, ATP production, glycolysis, osmolyte levels, and reactive oxygen species (ROS). Under hyperosmotic conditions, ALDH7A1 knockdown sensitized heated Huh-7 cells, while its overexpression promoted cell survival and invasion, with corresponding changes in energy metabolism and enzymatic products. Tan IIA and the specific ALDH7A1 inhibitor, 4-diethylaminobenzaldehyde, demonstrated similar effects on gene expression patterns, glycolysis, osmotic regulation, and ROS levels in heated Huh-7 cells. Moreover, Tan IIA is able to direct interact with ALDH7A1 protein. In vitro, Tan IIA combined with hyperosmotic stress significantly inhibited cell invasion and induced apoptosis in heat-induced Huh-7 cells and ALDH7A1 overexpression partially reversed the effects of Tan IIA. In vivo, Tan IIA combined with hyperosmotic stress or glycolysis inhibitor yielded better therapeutic efficacy for HCC. In conclusion, Tan IIA sensitizes HCC cells to sublethal heat by targeting ALDH7A1, leading to disrupted glycolytic and osmolytic balance, subsequently hindering tumor cell survival and increasing apoptosis. These findings highlight a potentially novel strategy for preventing or treating recurrent HCC post-thermal ablation using Tan IIA with hyperosmotic reagents.
2025-10-03 | GSE309428 | GEO