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Hemocytes and fat body cells, the only professional immune cell types in Drosophila, show strikingly different responses to systemic infection

ABSTRACT: Hemocytes and fat body cells, the only professional immune cell types in Drosophila, show strikingly different responses to systemic infection

PROVIDER: PRJNA863237 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR20690256.fastq.gz	Fastqsanger.gz
	SRR20690257.fastq.gz	Fastqsanger.gz
	SRR20690258.fastq.gz	Fastqsanger.gz
	SRR20690259.fastq.gz	Fastqsanger.gz
	SRR20690260.fastq.gz	Fastqsanger.gz

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Hemocytes and fat body cells, the only professional immune cell types in Drosophila, show strikingly different responses to systemic infection

Project description:The fruit fly Drosophila is an excellent model to study the response of different Q7 immunocompetent organs during systemic infection. In the present study, we intended to test the hypothesis that the only professional immune organs of the fly, the fat body and hemocytes, show substantial similarities in their responses to systemic infection. However, comprehensive transcriptome analysis of isolated organs revealed highly divergent transcript signatures, with the few commonly regulated genes encoding mainly classical immune effectors from the antimicrobial peptide family. The fat body and the hemocytes each have specific reactions that are not present in the other organ. Fat body-specific responses comprised those enabling an improved peptide synthesis and export. This reaction is accompanied by transcriptomic shifts enabling the use of the energy resources of the fat body more efficiently. Hemocytes, on the other hand, showed enhanced signatures related to phagocytosis. Comparing immune-induced signatures of both cell types with those of whole-body responses showed only a minimal correspondence, mostly restricted again to antimicrobial peptide genes. In summary, the two major immunocompetent cell types of Drosophila show highly specific responses to infection, which are closely linked to the primary function of the respective organ in the landscape of the systemic immune response.

2022-11-14 | GSE209960 | GEO

Hemocytes and fat body cells, the only professional immune cell types in <i>Drosophila</i>, show strikingly different responses to systemic infections.

Project description:The fruit fly Drosophila is an excellent model to study the response of different immunocompetent organs during systemic infection. In the present study, we intended to test the hypothesis that the only professional immune organs of the fly, the fat body and hemocytes, show substantial similarities in their responses to systemic infection. However, comprehensive transcriptome analysis of isolated organs revealed highly divergent transcript signatures, with the few commonly regulated genes encoding mainly classical immune effectors from the antimicrobial peptide family. The fat body and the hemocytes each have specific reactions that are not present in the other organ. Fat body-specific responses comprised those enabling an improved peptide synthesis and export. This reaction is accompanied by transcriptomic shifts enabling the use of the energy resources of the fat body more efficiently. Hemocytes, on the other hand, showed enhanced signatures related to phagocytosis. Comparing immune-induced signatures of both cell types with those of whole-body responses showed only a minimal correspondence, mostly restricted again to antimicrobial peptide genes. In summary, the two major immunocompetent cell types of Drosophila show highly specific responses to infection, which are closely linked to the primary function of the respective organ in the landscape of the systemic immune response.

| S-EPMC9726733 | biostudies-literature

Expression from hemocytes misexpressing Idh-R195H vs. controls

Project description:Expression profile for hemocytes from hml-Gal4, UAS-2xEGFP larvae were compared to hemocytes from hml-Gal4, UAS-2xEGFP; UAS-Idh-R195H larvae

2014-10-03 | GSE62008 | GEO

Transcriptomic analysis of gene expression in adult Drosophila hemocytes

Project description:Hemocytes are phagocytic blood cells that act as the first line of defense against bacterial pathogens in the fruit fly, Drosophila melanogaster. To gain insight into the immune-regluated transcriptional response in this cell type, we sequenced uninfected, mock (PBS) infected, and Staphylococcus aureus infected hemocytes collected from adult flies. Wildtype and A2bp1 RNAi hemocytes expressing a mouse-CD8-GFP fusion protein were isolated using immunselection and sequenced using 50bp single end reads on an Illumina HiSeq platform. A2bp1 RNAi hemocytes express the TRiP short hairpin RNA HMS00478 (which targets all isoforms of Ataxin-2 binding protein 1) under the hemolectinΔGAL4 promoter.

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Transcription profiling of Drosophila third instar larvae hemocytes TO EXPLAIN hemocyte development.

Project description:We measured gene expression profiles of third instar larvae hemocytes from selected strains related to hemocyte development. Every strain was purchased from commercial companies. And we used microarray data to find what biological processes show abnormalities first, and which gene expressions show similarities and differences between selected strains. Experiment Overall Design: 200~300 Drosophila third instar larvae hemocytes from each of five lines were dissected to get at least 1 microgram of total RNA. Because all samples were hybridized to single-dye Affymetrix chip, control data were compared with data from each mutant.

2008-04-05 | E-GEOD-10853 | biostudies-arrayexpress

Transcriptome profiling wild-type and Nurf301 mutant hemocytes

Project description:The purpose of the experiment was to define transcriptional targets of the nucleosome remodelling factor (NURF) chromatin remodelling enzyme in primary hemocytes. Hemocytes isolated from Drosophila wild-type and Nurf301[2] homozygous mutant 3rd instar hemocytes were compared by microarray analysis.

2016-04-05 | E-MTAB-4537 | biostudies-arrayexpress

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Project description:Expression profile for hemocytes from hml-Gal4, UAS-2xEGFP larvae were compared to hemocytes from hml-Gal4, UAS-2xEGFP; UAS-Idh-R195H larvae We sought to determine which genes and pathways were upregulated or downregulated in hemocytes expressing Idh-R195H, the Drosophila homolog of the human cancer-associated IDH1-R132H mutant, in vivo. We sought to obtain homogeneous populations of hemocytes from each genotype. We compared larvae expressing Idh-R195H from the hml-Gal4 driver to larvae with the hml-Gal4 driver alone.

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Gene Expression Profiles of Drosophila third instar larvae hemocytes to explain hemocyte development.

2008-03-18 | GSE10853 | GEO

SHOW-Me

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The gene expression profiles in response to fungal infection in hemocytes.

Project description:The object of the microarray data is to find several genes effected by fungal infection. In order to establish the gene expressino levels in response to fungal infection in hemocytes, B.bassiana were injected into the posterior end of third instar larvae using a tungsten needle and incubated for 1h at 25 celcius degree. To compare with non-infected hemocytes, total RNA from infected hemocytes were labeled by Cy5, total RNA from non-infected hemocytes were labeled by Cy3, and hybridized to chip.

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