Ontology highlight
ABSTRACT:
PROVIDER: PRJNA863409 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR20705600_1.fastq.gz | Fastqsanger.gz | |||
| SRR20705600_2.fastq.gz | Fastqsanger.gz | |||
| SRR20705601_1.fastq.gz | Fastqsanger.gz | |||
| SRR20705601_2.fastq.gz | Fastqsanger.gz | |||
| SRR20705602_1.fastq.gz | Fastqsanger.gz |
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Project description:Biotic and abiotic stresses limit agricultural yields, and plants are often simultaneously exposed to multiple stresses. Combinations of stresses such as heat and drought or cold and high light intensity, have profound effects on crop performance and yeilds To analyze such responses, we initially compared transcriptome changes in ten Arabidopsis thaliana ecotypes using cold, heat, high light, salt and flagellin treatments as single stress factors or their double combinations.
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Project description:Potato, the most important non-cereal crop, is highly water and space efficient but susceptible to abiotic stress such as heat, drought, or flooding. Due to climate change the likelihood of such stresses to occur, individually, sequentially, or simultaneously, has dramatically increased. While we have detailed insights into plant responses to single stresses in some model plants, the understanding of acclimation to multiple abiotic stresses in crops is still very limited. Here we present a comprehensive molecular and physiological profiling of potato (Solanum tuberosum, cv. Desirée) under both single and multiple abiotic stresses (heat, drought, and waterlogging), designed to mimic realistic future scenarios. Daily phenomics analysis enabled monitoring of the acclimation responses over time. In parallel, leaf samples were taken throughout the 28-day experiment for multi-omics analysis including transcriptomics, metabolomics, proteomics, and hormonomics. At the end of the period, critical metabolites of tuber samples were analysed. To facilitate the multi-omics analyses, the dataset was integrated with expert knowledge, an approach that is increasingly necessitated in the development of high-throughput pipelines in agricultural research. Waterlogging had immediate and most dramatic effects, and surprisingly, the responses were similar to drought stress. In contrast, distinct stress signatures at morpho-physiological, metabolic, and molecular levels were found in response to heat or drought stress and to a combination of both.
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Project description:Plants will meet various abiotic stresses during their growth and development. One of the important strategies for plants to deal with the stress is involved in metabolic regulation, causing the dramatic changes of metabolite profiles. Metabolomic studies have been intensively conducted to reveal the responses of plants to abiotic stress, but most of them were limited to one or at most two abiotic stresses in a single experiment. In this study, we compared the metabolite profiles of barley seedlings exposed to seven abiotic stresses simultaneously, including drought, salt stress, aluminum (Al), cadmium (Cd), deficiency of nitrogen (N), phosphorus (P) and potassium (K). The results showed that metabolite profiles of barley under these stresses could be classified into three types: osmotic stresses (drought and salt); metal stresses (Al and Cd) and nutrient deficiencies (N, P and K deficiencies). Compared with the control, some metabolites (including polyamines, raffinose and piperonic acid) in plants exposed to all abiotic stresses changed significantly, while some other metabolites showed the specific change only under a certain abiotic stress, such as proline being largely increased by osmotic stress (drought and salinity), the P-containing metabolites being largely decreased under P deficiency, some amino acids (lysine, tyrosine, threonine, ornithine， glutamine and so on) showing the dramatic reduction in the plants exposed to N deficiencies, respectively. The current meta-analysis obtained a comprehensive view on the metabolic responses to various abiotic stress, and improved the understanding of the mechanisms for tolerance of barley to abiotic stress.
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