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Effect of ILB on Gene expression in SHSY5Y neuroblastoma cells

ABSTRACT: Effect of ILB on Gene expression in SHSY5Y neuroblastoma cells

PROVIDER: PRJNA863474 | ENA |

REPOSITORIES: ENA

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Lamin A/C is required for SHSY5Y neuroblastoma cell differentiation and its expression is linked to a less malignant phenotype.

Project description:The main scientific objective of the project was to investigate whether Lamin A/C could be involved in neuroblastoma differentiation. Moreover, taking into account the significance of differentiation stage in the neuroblastoma tumor progression we have also studied a possible role of Lamin A/C in the tumorigenesis of this neuronal cancer. As differentiating stimulus we used the all-trans retinoic acid (RA), the most effective compound which has been shown to induce differentiation in neuroblastoma cells. To get insight into the impairment of cell differentiation produced by the LMNA (Lamin A/C) silencing in SHSY5Y cells, we compared the gene expression profile of control and silenced cells both in Retinoic Acid treated and untreated samples, using the one-color Agilent microarray platform.

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Project description:TDP-43 iCLIP of SHSY5Y cells

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Gene expression profiling of SHSY5Y cells exposed to Endosulfan

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Effect of ILB on Gene expression in SHSY5Y neuroblastoma cells

Project description:The experiments were carried out to further understand the mechanism of action of ILB, a low molecular weight dextran sulphate, in neuronal cell. The experiments were carried out with ILB concentrations that are relevant to the therapeutic concentrations of the drug. The results indicate that ILB regulates the expression of genes to enhance cell survival and differentiation is a dose dependent manner. Upstream regulator analysis indicates that the gene expression changes are due to enhanced activity of growth factors and cytokines.

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