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RNA-seq and miRNA microarray of control and METTL14-overexpressed HT29 human colon cancer cells

ABSTRACT: RNA-seq and miRNA microarray of control and METTL14-overexpressed HT29 human colon cancer cells

PROVIDER: PRJNA863577 | ENA |

REPOSITORIES: ENA

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Gene expression profiling by miRNA microarray of control and METTL14-overexpressed HT29 human colon cancer cells

Project description:In order to define the cohort of miRNAs whose expression is regulated by METTL14 in p53 mutant colorectal cancer, we constructed HT29 cells stably infected with lentivirus carrying control transfectants (vector) or METTL14 overexpression (METTL14). Subsequently, total RNA was isolated and then performed miRNA Microarray.

2023-03-27 | GSE210044 | GEO

Gene expression profiling by miRNA microarray of control and METTL14-overexpressed HT29 human colon cancer cells

Project description:Gene expression profiling by miRNA microarray of control and METTL14-overexpressed HT29 human colon cancer cells

| PRJNA863581 | ENA

Gene expression profiling by RNA-seq of control and METTL14-knockdown HT29 human colon cancer cells

Project description:In order to define the cohort of genes whose expression is regulated by METTL14 in p53 mutant colorectal cancer, we constructed HT29 cells stably infected with lentivirus carrying control shRNA (shNC) or METTL14 shRNA (shMETTL14). Subsequently, total RNA was isolated and then performed transcriptome RNA sequence.

2023-03-27 | GSE210045 | GEO

RNA-seq and miRNA microarray of control and METTL14-overexpressed HT29 human colon cancer cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-03-27 | GSE210056 | GEO

Analysis of microRNA expression through microarray scan in HCT116 human colon cancer cells with or without METTL14 overexpression

Project description:To reveal the changes of miRNAs levels in HCT116 cells with METTL14 overexpression, we employed miRNA microarray expression profiling in HCT116 human colon cancer cells stably expressing control vector or METTL14.

2023-03-27 | GSE186790 | GEO

Gene expression profiling by RNA-seq of control and METTL14-knockdown HT29 human colon cancer cells

Project description:Gene expression profiling by RNA-seq of control and METTL14-knockdown HT29 human colon cancer cells

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Transcriptome analysis of novel CPT-CEF-treated HT29 colon cancer cells to evaluate drug cytotoxicity

Project description:Purpose: To identify differentially expressed genes in HT29 colon cancer cells after treatment with a novel formulation of camptothecin with β-cyclodextrin-EDTA-Fe3O4 nanoparticle-conjugated nanocarriers (CPT-CEF) Methods:Treated HT29 cell lines with CPT-CEF, isolated total RNA from HT29 colon cancer cells, and prepared library for RNA sequencing. Carried out comparative transcriptomic studies between treated and untreated cells to find out which gene functions were dysregulated by CPT-CEF. Results: The study yielded 247 DEGs ((FDR<0.05, FC>2.0) that were affected by CPT-CEF treatment in the HT29 colon cancer cells. The results obtained from cell cycle analysis, mitochondrial depolarization assay and acridium orange/propidium iodide double staining showed potential of CPT-CEF in cancer cell inhibition. Conclusion: Our study successfully identified DEGs in the CPT-CEF treated HT29 colon cancer cells that pointed to inhibition of cancer progression. To further affirm, animal studies are needed.

2021-09-15 | GSE165875 | GEO

Diverse effect of WWOX overexpression in HT29 colon cancer cell line.

Project description:The WWOX gene is a tumor suppressor probably involved in regulation of cell cycle and apoptosis and downregulated in variety of cancer types.However, its role in colon cancerogenesis is unknown. The aim of this study was to characterize how WWOX may be involved in colon cancerogenesis or cancer progression, how it influences the basic cancer cell features and modifies cell expression profile.Our observations suggest that in HT29 colon cancer cell line increased expression of WWOX may result in transition of cancer cells into more normal- like colon epithelium phenotype, on the other hand in SW480 WWOX revealed the well-known tumour suppressor properties. However, as the colon cancer is very heterogeneous disease, obtained discrepancies may reflect the known differences between cell lines and cancerogenesis pathway, which they undergone. HT29 colon cancer cells were stably transfected with WWOX cDNA. HT29 cells transfected with an empty vector served as a control. Total mRNA was isolated to look for gene-expression differences induced by the WWOX overexpression.

2014-01-23 | E-GEOD-54298 | biostudies-arrayexpress

HCT116 human colon cancer cells and METTL14

Project description:HCT116 human colon cancer cells and METTL14

| PRJNA776018 | ENA

ERbeta modulation of NFkB pathway in colon cancer cell lines SW480 and HT29

Project description:Analysis of differentially expressed genes in colon cancer cell lines SW480 and HT29 with and without stably expressed ERbeta gene, with and without 10ng/mL TNFa treatment for 2 and 24 hours. Total RNA obtained from colon cancer cell lines SW480 and HT29 with and without stably expressed ERbeta gene, with and without 10ng/mL TNFa treatment for 2 and 24 hours.

2016-07-17 | E-GEOD-65979 | biostudies-arrayexpress

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